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High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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Immunology and Infection
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JoVE Journal Immunology and Infection
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

DOI:
10.3791/51222-v

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11:34 min

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May 05, 2014

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, , , , , ,
1Unité de Génomique Virale et Vaccination, Virology Department,Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department,Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department,Institut Pasteur

Chapters

  • 00:05Title
  • 01:34Preparation of 96-well Daughter Plates with Compounds
  • 03:03Preparation of Cell Cultures to Determine Compound Toxicity
  • 04:18Preparation of Cell Cultures Infected by rMV2/Luc
  • 05:37Luciferase Activity Measurement
  • 06:36Secondary Screening for Toxicity and Broad-spectrum Antiviral Activity
  • 08:52Results: Identification of Anti-viral Hit Compounds
  • 11:06Conclusion

Summary

Automatic Translation

Automatic Translation

In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.

Tags

Keywords: High-throughput ScreeningBroad-spectrum Chemical InhibitorsRNA VirusesCellular PathwaysHost Cell FunctionsAntiviral CompoundsRecombinant VirusesReporter EnzymesBioluminescenceLuciferaseMeasles VirusChikungunya VirusCell Culture Assays

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