Chorion Softening

Chorion softening is the biological process in which the protective protein-rich envelope surrounding an egg becomes more flexible and easier to breach, enabling embryonic development or hatching. It commonly occurs when enzymes released by the developing embryo or surrounding tissues hydrolyze structural proteins in the chorion, weakening its organized matrix and reducing mechanical resistance. In biology, studying this process helps explain how embryos escape their protective coverings and how hatching is timed in aquatic species. These mechanisms also support research in embryology, reproductive biology, and aquaculture, where chorion integrity can influence embryo survival, development, and successful emergence.

Chorion Softening - Related Videos

Research

JoVE EoE - Drosophila melanogaster (fruit fly)

Chorion and Vitelline Membrane Mechanical Removal: A Method to Prepare Drosophila Oocytes for Direct Observation

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2023

This video describes a method to mechanically remove the chorion and vitelline membranes from previously fixed mature Drosophila oocytes. The featured protocol shows a detailed demonstration of the procedure yielding oocytes compatible with fluorescent in situ hybridization assays.

Environmental Dynamic Mechanical Analysis to Predict the Softening Behavior of Neural Implants

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Cited by 4 •

2019

To allow reliable predictions of the softening of polymeric substrates for neural implants in an in vivo environment, it is important to have a reliable in vitro method. Here, the use of dynamic mechanical analysis in phosphate buffered saline at body temperature is presented.

Research

JoVE Journal - Developmental Biology
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Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

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Cited by 91 •

2016

Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

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Cited by 5 •

2015

This protocol provides a simple and efficient way to propagate human pluripotent stem cells (hPSCs) using only conditioned media derived from the human placenta in a gelatin-coated dish without additional exogenous supplementation or hPSC-specific synthetic substrata.

Ultrastructural Analysis of a Mouse Brain Section Using Transmission Electron Microscopy

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2025

Source: Lutz, D., et.al. Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord. J. Vis. Exp. (2019).This video demonstrates the preparation and transmission electron microscopy (TEM) analysis of a brain section from a mouse pup. The protocol starts with osmium tetroxide-fixed tissue embedded in resin, followed by precise trimming to isolate the region of interest and ultramicrotomy to produce semithin and ultrathin...

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