Oxygen Mapping
Oxygen Mapping - Related Videos
09:40
Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
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Cited by 3 •
2010
This protocol describes a method for micron-scale three-dimensional imaging of oxygen concentration in the immediate environment of live cells by electron spin resonance microscopy.
11:03
Motor Maps
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2023
Source: Laboratories of Jonas T. Kaplan and Sarah I. Gimbel—University of Southern California One principle of brain organization is the topographic mapping of information. Especially in sensory and motor cortices, adjacent regions of the brain tend to represent information from adjacent parts of the body, resulting in maps of the body expressed on the surface of the brain. The primary sensory and motor maps in the brain surround a prominent sulcus known as the central sulcus. The cortex...
06:58
Fate Mapping
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2023
Fate mapping is a technique used to understand how embryonic cells divide, differentiate, and migrate during development. In classic fate mapping experiments, cells in different areas of an embryo are labeled with a chemical dye and then tracked to determine which tissues or structures they form. Technological improvements now allow for individual cells to be marked and traced throughout embryonic development and adulthood. This video reviews the concepts behind fate mapping, and then details a...
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 27 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
11:35
Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function
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Cited by 16 •
2010
A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.
FAQs
The indicator converts local oxygen interactions into measurable changes in fluorescence, phosphorescence, or signal intensity. Recording those changes at different positions across a sample allows researchers to estimate oxygen levels spatially, then visualize gradients instead of collapsing the tissue into one average value. This preserves information about local biological microenvironments.
Local oxygen patterns reflect the combined effects of diffusion, cellular respiration, and blood flow. These processes do not distribute oxygen uniformly, so neighboring regions can experience different availability. Oxygen mapping makes this heterogeneity visible, helping researchers relate oxygen conditions to tissue organization, cellular activity, and changing microenvironmental conditions.
A spatially resolved map can reveal gradients and localized regions that an overall oxygen value could hide. This distinction matters when studying biological systems with uneven oxygen availability, because different areas may experience different metabolic or functional conditions. The resulting patterns support more precise interpretation of hypoxia, tissue behavior, and cellular responses.
Researchers first apply or use an oxygen-sensitive probe, dye, or sensor with the sample, tissue, or cellular environment. They then measure the indicator's fluorescence, phosphorescence, or signal intensity across locations and convert those spatial measurements into an oxygen visualization. The resulting map can be examined for local levels and gradients.
The approach can be applied to tumors, developing tissues, organoids, and engineered cultures. These systems are useful because oxygen availability may vary within them as diffusion, respiration, and blood flow shape their environments. Mapping those differences helps investigators study how oxygen conditions relate to development, tissue function, and disease-related changes.
Oxygen mapping supports investigations of metabolism, hypoxia, tissue function, and disease progression. It can also show how cells respond when oxygen conditions change across their environment. By connecting measured oxygen patterns with these biological processes, researchers gain context for interpreting local cellular behavior rather than treating the sample as chemically uniform.