Collagenase Solution
Collagenase Solution - Related Videos
07:09
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Cited by 93 •
2013
A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.
09:41
Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
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Cited by 35 •
2014
Preclinical models of intracerebral hemorrhage are utilized to mimic certain aspects of clinical disease. Thus, mechanisms of injury and potential therapeutic strategies may be explored. In this protocol, two models of intracerebral hemorrhage are described, intrastriatal (basal ganglia) injections of autologous blood or collagenase.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
06:59
Mouse Islet of Langerhans Isolation using a Combination of Purified Collagenase and Neutral Protease
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Cited by 103 •
2012
A detailed description of mouse islet isolation is described using the technique of in situ pancreatic ductal cannulation and perfusion of a combination of purified collagenase and neutral protease.
FAQs
These variables determine how rapidly and extensively the extracellular matrix is loosened. Higher concentration or longer exposure can increase tissue disruption, while temperature and tissue type alter how readily collagen is cleaved. Researchers therefore adjust the combination rather than treating any single setting as universal, seeking enough digestion to release cells without overprocessing the sample.
Excessive treatment can reduce cell viability and damage cell-surface proteins, creating a cell preparation that is less suitable for downstream culture or behavioral analysis. The problem is not simply loss of structural collagen; prolonged or overly strong digestion can alter the biological material being studied. Controlled exposure helps preserve both living cells and relevant surface features.
Insufficient digestion leaves portions of the extracellular matrix intact, so embedded cells may remain trapped and tissue disruption may be incomplete. This can reduce the number of cells released and produce a less representative preparation for culture or analysis. The result reflects an underprocessed sample rather than necessarily a biological absence of the cells of interest.
A practical workflow begins by matching the reagent conditions to the tissue, then selecting concentration, temperature, and exposure time for controlled digestion. Researchers assess whether the tissue loosens enough to release embedded cells while avoiding excessive treatment. The resulting preparation can support primary-cell isolation, culture preparation, or examination of cell behavior.
It is especially useful when researchers need to obtain primary cells from intact tissue rather than study only an already separated cell population. Dissociation can prepare material for cell culture and enable direct examination of cell behavior after release from the surrounding matrix. This makes the reagent relevant to experiments that require cells originating from a specific tissue.
By loosening the surrounding matrix, the method can provide a physiologically relevant starting context for studying cells from organized tissue. Researchers can examine behavior after release while considering how digestion may influence viability and surface proteins. Interpretation therefore requires attention to both the cells' tissue origin and the processing conditions used to obtain them.