Micro Pipette Preparation
Micro Pipette Preparation - Related Videos
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
09:48
Neural Activity Propagation in an Unfolded Hippocampal Preparation with a Penetrating Micro-electrode Array
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Cited by 2 •
2015
We have developed an in vitro unfolded hippocampus which preserves CA1-CA3 array of neurons. Combined with the penetrating micro-electrode array, neural activity can be monitored in both the longitudinal and transverse orientations. This method provides advantages over hippocampal slice preparations as the propagation in the entire hippocampus can be recorded simultaneously.
10:54
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
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Cited by 10 •
2019
We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.
05:12
Pressure-polishing Pipettes for Improved Patch-clamp Recording
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Cited by 13 •
2008
This is a guide to modifying the shape of glass micropipettes. Specifically, by using heat and air pressure the taper is widened without increasing the tip opening, leading to lower pipette resistance. This is critical to obtain low noise recordings of small cells but is useful in many applications.
FAQs
Tip dimensions influence three practical behaviors: fluid flow, sample uptake, and the force needed to manipulate material. A finer or differently shaped tip therefore changes how readily a sample enters or leaves the pipette and how much mechanical effort a task requires. Matching dimensions to the intended manipulation helps limit sample damage and supports more reproducible biological experiments.
Heating softens the glass capillary so it can be pulled into a tapered form. The resulting taper depends on how the glass responds during this preparation stage, making consistent heating important for producing comparable pipettes. If the formed tips vary substantially, fluid handling and the force required for manipulation may also vary, reducing experimental consistency.
Pulling creates the initial taper, while trimming or shaping prepares the end for its intended biological use. This finishing step helps establish the usable tip geometry that governs sample entry, fluid delivery, and interaction with cells or tissues. Careful shaping can therefore support controlled manipulation during injection, aspiration, patch clamping, or microdissection.
Preparation begins with a fine glass capillary, which is heated until the glass softens and then pulled to create a tapered tip. The tip is subsequently trimmed or shaped, and the pipette is prepared for use. Maintaining consistency across these stages helps produce tools with comparable dimensions and predictable handling characteristics in biological experiments.
This preparation supports biological procedures that require precise handling or delivery of small volumes. Applications identified for the technique include cell injection, aspiration, patch clamping, and microdissection. It is relevant to work with cells, embryos, and tissues, particularly in developmental, cellular, and physiological research where controlled manipulation can affect sample integrity and experimental reproducibility.
Consistent preparation reduces variation in tip dimensions, which helps stabilize fluid flow, sample uptake, and the force applied during manipulation. That consistency can reduce sample damage and make results more reproducible across experiments. In studies involving cells, embryos, or tissues, standardized pipettes support more controlled handling and clearer interpretation of biological responses.