Electron Donors
Electron Donors - Related Videos
07:17
Single Port Donor Nephrectomy
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Cited by 5 •
2011
Single port laparoscopic surgery is changing the standard of care in surgical care like nothing since the laparoscopic technique was introduced 20 years ago. We present out technique of single port donor nephrectomy using the Gelpoint device. We have successfully performed this surgery in 100 patients.
12:13
Ex Situ Normothermic Machine Perfusion of Donor Livers
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Cited by 21 •
2015
Here we present a protocol describing oxygenated ex situ machine perfusion of donor liver grafts. This article contains a step by step protocol to procure and prepare the liver graft for machine perfusion, prepare the perfusion fluid, prime the perfusion machine and perform oxygenated normothermic machine perfusion of the liver graft.
08:37
Corneal Donor Tissue Preparation for Endothelial Keratoplasty
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Cited by 31 •
2012
Endothelial corneal transplantation is a surgical technique for treatment of posterior corneal diseases. Mechanical microkeratome dissection to prepare tissue results in thinner, more symmetric grafts with less endothelial cell loss and improved outcomes. Dissections can be performed at the eye bank prior to corneal transplantation surgery.
12:52
Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA First discovered by Lederberg and Tatum in 1946, conjugation is a form of horizontal gene transfer between bacteria that relies on direct physical contact between two bacterial cells (1). Unlike other forms of gene transfer, such as transformation or transduction, conjugation is a naturally occurring process in which...
10:22
Enrichment of Bruch's Membrane from Human Donor Eyes
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Cited by 14 •
2015
Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.
FAQs
Reduced molecules such as NADH transfer electrons through enzyme-catalyzed reactions or electron transport chains. As electrons move between reaction partners, the process can establish a proton gradient across a membrane. Cells then use that gradient to support ATP production, linking the chemical energy in donor molecules to a form that powers cellular activities.
Electron donors differ in their chemical properties and availability, so organisms access them through distinct metabolic pathways. NADH and organic substrates commonly participate in cellular reactions, whereas hydrogen or reduced sulfur compounds can support specialized microbial metabolisms. These differences help determine which organisms thrive under particular conditions and how communities partition energy resources.
The supply of suitable electron donors can shape microbial community structure by favoring organisms able to use the available compounds. Their consumption connects metabolism with nutrient cycling and energy flow through ecosystems. Changes in donor availability may therefore alter which biochemical processes dominate, influencing interactions among microbes and the movement of biologically important elements.
Respiration and photosynthesis research examines how electron transfer links donor molecules to cellular energy conversion. In these systems, researchers can evaluate how compounds such as NADH, organic substrates, hydrogen, or reduced sulfur compounds participate in enzyme-catalyzed pathways. This perspective helps relate molecular redox reactions to proton-gradient formation and broader metabolic outcomes.
They are particularly important when investigating anaerobic metabolism, because organisms operating without the same conditions as oxygen-dependent respiration still require electron-transfer pathways to conserve energy. Hydrogen, reduced sulfur compounds, and organic substrates can serve as relevant inputs for these pathways. Studying their use clarifies microbial energy strategies and their effects on nutrient cycling.
In bioremediation, donor chemistry helps researchers understand microbial processes that transform compounds in affected environments. In engineered biological energy systems, donor selection influences how efficiently biological reactions connect electron transfer with energy production. Examining donor availability and chemical properties can guide interpretation of system performance, microbial activity, and resulting changes in energy flow.