Embryo Explantation
Embryo Explantation - Related Videos
09:40
Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
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Cited by 6 •
2015
This protocol describes a method to dissect, experimentally manipulate and culture whole retinal explants from chicken embryos. The explant cultures are useful when high success rate, efficacy and reproducibility are needed to test the effects of plasmids for electroporation and/or reagent substances, i.e., enzymatic inhibitors.
09:47
Isolation of Cerebrospinal Fluid from Rodent Embryos for use with Dissected Cerebral Cortical Explants
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Cited by 20 •
2013
The ventricular cerebrospinal fluid (CSF) bathes the neuroepithelial and cerebral cortical progenitor cells during early brain development in the embryo. Here we describe the method developed to isolate ventricular CSF from rodent embryos of different ages in order to investigate its biological function. In addition, we demonstrate our cerebral cortical explant dissection and culture technique that allows for explant growth with minimal volumes of culture medium or CSF.
06:25
Explant Culture for Developmental Studies
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2023
Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo approach allows researchers to manipulate and observe developing tissues in ways that are not possible in vivo. Once established, explant culture is frequently used to understand the role of genes and signaling molecules in organogenesis. This video will first introduce the basic principles of explant culture and demonstrate a protocol to...
07:45
Explant Culture of Neural Tissue
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2023
The intricate structure of the vertebrate nervous system arises from a complex series of events involving cell differentiation, cell migration, and changes in cell morphology. Studying these processes is essential to our understanding of nervous system function as well as our ability to diagnose and treat disorders that result from abnormal development. However, neural tissues are relatively inaccessible for experimental manipulations, especially in embryonic mammals. As a result, many...
07:21
Generation of Naïve Blastoderm Explants from Zebrafish Embryos
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Cited by 5 •
2021
Zebrafish blastoderm explants are generated by isolating embryonic cells from endogenous signaling centers within the early embryo, producing relatively naïve cell clusters easily manipulated and cultured ex vivo. This article provides instructions for making such explants and demonstrates their utility by interrogating roles for Nodal signaling during gastrulation.
FAQs
The nutrient medium provides the support needed for an isolated embryo to remain viable and continue growing outside its original tissues. Depending on the experimental purpose, it may also support regeneration into a complete plant. Observing whether embryos survive, develop, or regenerate helps researchers evaluate the suitability of culture conditions and the biological requirements of embryonic growth.
Separating the embryo from surrounding maternal tissues allows researchers to examine development under controlled laboratory conditions rather than within the original reproductive structure. This isolation makes it possible to investigate how nutritional and hormonal requirements affect embryonic growth. The approach therefore links developmental outcomes to selected culture conditions and reduces the influence of tissues that would otherwise remain associated with the embryo.
Researchers can culture isolated embryos under controlled conditions while examining how nutritional and hormonal support affects survival, growth, or regeneration. These outcomes provide experimental evidence about which requirements are important during development. Because the embryo is separated from its original tissues, changes observed in culture can be related more directly to the conditions being studied.
Assessment may focus on whether the isolated embryo survives, continues growing, or regenerates into a complete plant. These outcomes provide different levels of biological information, from basic viability to the capacity for organized plant development. Comparing such responses under laboratory conditions helps researchers study embryonic development and determine whether culture conditions support recovery or further growth.
A typical workflow begins with aseptically removing the embryo from a seed or another reproductive structure. The isolated material is then placed on a nutrient medium and maintained under controlled laboratory conditions. Researchers monitor survival, growth, and, where relevant, regeneration into a complete plant. This sequence connects careful isolation with subsequent culture-based observation.
Embryo rescue is useful when seeds fail to mature normally but the embryo may still be recoverable. Culturing the embryo separately can provide conditions that support its survival and development, potentially allowing a plant to be obtained despite unsuccessful seed maturation. This makes the technique relevant to recovering valuable plant material that might otherwise be lost.
In plant breeding, the technique can help recover valuable hybrid plants whose seeds may not mature normally. It also supports the study or propagation of plants that are otherwise difficult to propagate, provided the isolated embryo responds successfully to culture. The resulting plants and developmental responses can therefore contribute both to breeding objectives and to biological investigations of plant regeneration.