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JoVE Journal
Developmental Biology

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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time
 

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Article doi: 10.3791/56214
August 9th, 2017

Summary August 9th, 2017

A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.

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