Embryonic Day 12.5
Embryonic Day 12.5 - Related Videos
10:51
A System for ex vivo Culturing of Embryonic Pancreas
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Cited by 23 •
2012
Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
10:49
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
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2022
In this protocol, we describe how to inject the mouse amniotic cavity at E7.5 with lentivirus, leading to uniform transduction of the entire neural plate, with minimal detrimental effects on survival or embryonic development.
07:23
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
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Cited by 5 •
2016
A detailed procedure for the synthesis of a 125I-labeled azide and the radiolabeling of dibenzocyclooctyne (DBCO)-group-conjugated, 13-nm-sized gold nanoparticles using a copper-free click reaction is described.
06:56
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
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Cited by 2 •
2021
We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.
00:58
Embryonic Stem Cells
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2019
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease. Origin ES cells are present in the inner cell mass of an embryo at the blastocyst stage, which occurs at about 3–5 days after fertilization in humans before the embryo is implanted in the uterus.
FAQs
Using a defined embryonic age gives experiments a common temporal reference. In mouse studies, E12.5 lets investigators compare anatomy, molecular expression, and developmental outcomes at a matched point rather than relying only on later characteristics. This consistency is particularly valuable when assessing how altered genes or environmental conditions change otherwise comparable developmental processes.
At E12.5, organ development reflects several coordinated activities rather than a single cellular event. Proliferation expands cell populations, differentiation establishes specialized cell types, migration positions cells, and morphogenesis reshapes tissues. Studying these processes together helps investigators connect cellular behavior with the formation and refinement of organ structures during organogenesis.
Signaling interactions help establish and maintain distinct tissue identities during this stage. Their importance lies in coordinating neighboring tissues while cells proliferate, differentiate, migrate, and contribute to morphogenesis. Examining tissue identity alongside these cellular behaviors can reveal whether a developmental abnormality reflects disrupted signaling, altered cell behavior, or both.
An E12.5 analysis can bring together anatomical observations, molecular expression patterns, and developmental outcomes. Considering these readouts at the same embryonic timepoint helps relate visible organ structure to underlying molecular and cellular changes. The approach supports comparisons among experimental groups and helps identify developmental consequences associated with genetic or environmental perturbations.
Investigators can examine embryos at E12.5 after a genetic alteration and compare their anatomy, molecular expression, or developmental outcomes with observations from other experiments. Because the timepoint is defined, differences can be interpreted in relation to organogenesis and tissue refinement, helping connect gene activity with developmental processes without reducing the analysis to a single endpoint.
The stage captures a period when major structures undergo rapid growth and refinement, making developmental deviations potentially informative. Researchers can use E12.5 embryos to examine how genetic or environmental perturbations affect organ formation and tissue organization. Comparing resulting anatomy and molecular expression helps characterize developmental outcomes associated with congenital abnormalities.