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Journal / Developmental Biology / Cell Dissociation from the Tongue Epithelium and…
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Developmental Biology

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JoVE Journal Developmental Biology
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
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Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

Article DOI: 10.3791/62163-v • 06:56 min • January 21st, 2021
January 21st, 2021 •
1, 1, 1, 1
1Regenerative Bioscience Center; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences, University of Georgia

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Summary
Automatic Translation

We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.

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Cell Dissociation Tongue Epithelium Mesenchyme/connective Tissue Embryonic-day 12.5 8-week-old Mice High Yield Quality Cells Experimental Analysis Single Cell RNA Sequencing Primary Stem Cell Cultures Protocol Mammalian Tongue Euthanized Pregnant Female Mouse Surgical Area 70% Ethanol Operating Site Dissecting Scissors Uterine Horns Embryos Tyrode's Solution Dissecting Microscope Mini Scissors Fine Forceps Mouth Cavity Mandible Sterile Tyrode's Solution Enzyme Mixture Dispase And Collagenous
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