Endocytic Dynamics
Endocytic Dynamics - Related Videos
08:02
Live-cell Imaging of Endocytic Transport using Functionalized Nanobodies in Cultured Cells
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Cited by 1 •
2025
Endocytic and retrograde transport of proteins from the plasma membrane to the trans-Golgi network is essential to maintaining membrane homeostasis and regulating signaling. Here, we describe a method to image and quantify endocytic transport of transmembrane cargo proteins by live-cell microscopy using derivatized anti-GFP nanobodies in HeLa cells.
11:32
Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation
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Cited by 23 •
2009
Regulated endocytosis governs the cell surface expression levels of the majority of membrane proteins. Here we utilize reducible, membrane impermeant biotinylation reagents to measure the endocytic rate of the dopamine transporter (DAT), a polytopic membrane protein. The method facilitates a straightforward approach to measuring the endocytic rate of most plasma membrane proteins.
09:46
Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
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Cited by 20 •
2014
Several methods are available for measuring intracellular pH but very few of these allow simultaneous measurement of cytoplasmic and organellar pH. Here, we describe in detail a rapid and accurate methodology to simultaneously measure cytoplasmic and vesicular pH by ratiometric imaging of living cells.
05:13
Density Gradient Ultracentrifugation for Investigating Endocytic Recycling in Mammalian Cells
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Cited by 1 •
2021
This paper aims to present a protocol for preparing recycling endosomes from mammalian cells using sucrose density gradient ultracentrifugation.
12:03
Dynamics of Structures
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2023
Source: Roberto Leon, Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA It is rare nowadays that a whole year goes by without a major earthquake event wreaking havoc somewhere around the world. In some cases, like the 2005 Banda Ache earthquake in Indonesia, the damage involved large geographic areas and casualties in the six figures. In general, the number and intensity of earthquakes is not increasing, however, the vulnerability of the built environment is...
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Clathrin coats provide structural support as the plasma membrane bends, while adaptor proteins connect the coat to cargo and membrane components. Their coordinated action helps organize a budding region before vesicle scission. This division of labor is important because membrane deformation alone does not explain how particular extracellular material becomes concentrated in a forming vesicle.
Rab GTPases act as regulatory components across the trafficking pathway, coordinating vesicle movement, fusion, and endosome maturation. Their role links the timing of one event to the next: newly formed vesicles must move and fuse with early endosomes before sorting can direct their contents onward. Following these transitions helps explain how trafficking remains spatially and temporally organized.
Once material reaches early endosomes, sorting determines whether cargo recycles, undergoes degradation, or follows another route. These alternatives give endocytic dynamics different cellular consequences: recycling can support continued receptor or membrane availability, whereas degradation can reduce the presence of internalized material. The sorting decision therefore connects vesicle trafficking to signaling and membrane homeostasis.
To analyze the process, track the sequence from membrane bending through vesicle scission, delivery to early endosomes, and subsequent sorting. Then consider movement, fusion, and maturation as linked events rather than isolated steps. This sequence provides a framework for interpreting how cargo changes location and destination over time, even when the biological question concerns uptake or signaling.
By following cargo from internalization to its endosomal destination, researchers can relate trafficking behavior to receptor regulation and cellular signaling. The same framework also helps examine how pathogens enter cells, since entry can involve membrane remodeling and intracellular delivery. Comparing these outcomes reveals how a shared trafficking system supports normal communication and can also be exploited by infectious agents.
Trafficking defects are biologically important because disruptions in membrane internalization, vesicle delivery, sorting, or maturation can interfere with receptor regulation, nutrient uptake, signaling, and membrane homeostasis. Studying these links helps place disease-associated abnormalities within a cellular pathway rather than treating them as isolated molecular changes. Endocytic dynamics therefore connects altered transport with broader effects on cell function.