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In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

Article DOI: 10.3791/61716-v • 09:44 min • September 24th, 2020
September 24th, 2020 •
1,2, 1,3,4, 5,6,7, 1, 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

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Summary
Automatic Translation

This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described.

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In Vitro Evaluation Oncogenic Transformation Human Mammary Epithelial Cells Cell Model Time-consuming Tools Simple-to-use Tools Cell Transformation In Vitro Breast Primary Epithelial Cell Culture Supernatant Fetal Bovine Serum PBS Trypsin Centrifugation Fresh Medium Cell Concentration T25 Flask Population Doublings Proliferation Rate
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