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In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
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1Department of Cell Biology, Physiology and Immunology,Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities,IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology,IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell),IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine,University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences,University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology,University of Vic
Chapters
- 00:04Introduction
- 00:42Breast Primary Epithelial Cell Passaging and Population Doubling Calculation
- 02:12Three-Dimensional Cell Culture in Basement Membrane Matrix and Immunofluorescent Protein Detection
- 05:07Anchorage-Independent Assay and Colony Quantification
- 07:19Results: Representative BPEC Transformation Evaluation
- 09:15Conclusion
This protocol provides experimental in vitro tools to evaluate the transformation of human mammary cells. Detailed steps to follow-up cell proliferation rate, anchorage-independent growth capacity, and distribution of cell lineages in 3D cultures with basement membrane matrix are described.
