In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells

This methodology uses the integration of different indicators to assess the degree of transformation of any cell model. This technique provides researchers with a set of time-consuming but simple-to-use tools for assessing cell transformation in vitro. This allows the method to be used in most laboratories.

Demonstrating the procedure will be Teresa Anglada, a post-doctoral researcher from my laboratory, and Joan Repulles, a microscopy specialist. When the breast primary epithelial cell culture reaches 90%confluency, transfer the supernatant from the culture flask to a 15 milliliter tube containing two milliliters of fetal bovine serum and wash the cells with PBS. Treat the cells with one milliliter of trypsin for five minutes at 37 degrees Celsius.