Escherichia Coli Rp437
Escherichia Coli Rp437 - Related Videos
03:11
Preparation of Bioluminescent Enterohemorrhagic Escherichia coli Inoculum
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2025
Source: Kuo, C., et al. Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System. J. Vis. Exp. (2018)This video demonstrates the preparation of a luciferase-expressing enterohemorrhagic Escherichia coli suspension from frozen stock for use as an oral inoculum in animal models. This enables real-time, bioluminescent tracking of intestinal colonization during in vivo infection studies.
09:22
Amikacin Protection Assay for Quantification and Visualization of Escherichia coli Cell Invasion
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2026
Antibiotic resistance amongst adherent-invasive Escherichia coli strains poses challenges for assessing bacterial invasion using the traditional gentamicin protection assay. An improved method to evaluate bacterial invasion quantitatively by enumerating colony-forming units and qualitatively through visualization of infected cells by immunofluorescence confocal microscopy is described.
12:48
The Multifaceted Benefits of Protein Co-expression in Escherichia coli
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Cited by 4 •
2015
Protein co-expression is a powerful alternative to the reconstitution in vitro of protein complexes, and is of help in performing biochemical and genetic tests in vivo. Here we report on the use of protein co-expression in Escherichia coli to obtain protein complexes, and to tune the mutation frequency of cells.
10:34
Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
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Cited by 13 •
2017
Here we present a method for directly measuring transfer RNA charging levels from purified Escherichia coli RNA as well as a way to compare relative levels of transfer RNA, or any other short RNA, across different samples based on the addition of spike-in cells expressing a reference gene.
08:32
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
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2016
This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.
FAQs
Chemical signals first interact with methyl-accepting chemotaxis proteins, which regulate the activity of the CheA kinase. This changes the phosphorylation state of CheY, a signaling protein connected to the flagellar motor. The resulting change in motor behavior shifts cells between smooth runs and tumbles, allowing researchers to connect sensory input with observable movement.
CheA functions as a kinase whose activity is regulated through the chemotaxis signaling system. Its activity influences how much CheY becomes phosphorylated. Phosphorylated CheY then provides the signal that affects flagellar motor rotation. Measuring behavioral changes therefore gives researchers an indirect way to assess signaling through the CheA-CheY pathway.
Runs and tumbles provide visible behavioral outputs of intracellular signaling. A smooth run reflects one state of flagellar motor control, whereas a tumble reflects another. Tracking transitions between these states lets investigators quantify how cells respond to chemical signals and determine whether mutations alter the connection between sensing, signal transduction, and motility.
Researchers observe swimming cells with microscopy and can also use microfluidic systems to examine responses under controlled experimental conditions. Single-cell measurements follow movement or changes in run and tumble behavior rather than relying only on a population average. These approaches support quantitative comparisons of signaling responses, motility patterns, and chemotactic performance.
A mutant can be studied against the reproducible wild-type background provided by RP437. Researchers compare swimming responses, flagellar behavior, or chemotactic performance between the reference strain and the altered strain. Differences can then be interpreted in relation to disrupted sensing or signal-transduction components, helping identify how particular pathway changes affect bacterial behavior.
RP437 is particularly useful when experiments require a consistent reference for quantitative studies of bacterial sensing, motility, and flagellar behavior. Its established use supports comparisons across microscopy, microfluidic, and single-cell assays. By providing a common baseline, the strain helps researchers evaluate signaling mutants and interpret changes in chemotactic performance.