The Multifaceted Benefits of Protein Co-expression in <em>Escherichia coli</em>

Alessandra Stefan; Alessandro Ceccarelli; Emanuele Conte; Alejandro Mont&#243;n Silva; Alejandro Hochkoeppler

doi:10.3791/52431

Journal

/

Biology

/

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

The Multifaceted Benefits of Protein Co-expression in <em>Escherichia coli</em>

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

The Multifaceted Benefits of Protein Co-expression in Escherichia coli

11,946 Views

•

12:48 min

•

February 05, 2015

DOI:

10.3791/52431-v

DOI:

10.3791/52431-v

•

12:48 min

February 05, 2015

•

11926 Views

Alessandra Stefan^1,2, Alessandro Ceccarelli¹, Emanuele Conte¹, Alejandro Montón Silva¹, Alejandro Hochkoeppler^1,2

¹Department of Pharmacy and Biotechnology,University of Bologna ²CSGI, Department of Chemistry,University of Firenze

Summary

Protein co-expression is a powerful alternative to the reconstitution in vitro of protein complexes, and is of help in performing biochemical and genetic tests in vivo. Here we report on the use of protein co-expression in Escherichia coli to obtain protein complexes, and to tune the mutation frequency of cells.