Extravasation
Extravasation - Related Videos
09:00
Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
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Cited by 6 •
2017
Here, we describe a human blood-brain barrier model enabling to investigate lymphocyte transmigration into the central nervous system in vitro.
10:56
A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
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Cited by 7 •
2013
The three-dimensional flow chamber device is a novel in vitro technology for the quantitative and step-wise evaluation of the extravasation cascade of cells circulating under conditions of physiological shear stress. The device therefore fills a critical need for basic, preclinical, and clinical studies of cell migration.
02:49
A Technique to Assess the Immune Response against an Antigen by Inducing Skin Inflammation
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2025
This video demonstrates an in vivo technique to assess a test antigen by inducing skin inflammation in a murine model. The test antigen—a hapten in an oil/acetone solution—is topically applied to the skin of the mouse ear, and inflammation of the skin is assessed after an adequate period.
07:31
Testing the Vascular Invasive Ability of Cancer Cells in Zebrafish (Danio Rerio)
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Cited by 19 •
2016
This method utilizes zebrafish embryos to efficiently test the vascular invasive ability of cancer cells. Fluorescent cancer cells are injected into the precardiac sinus or yolk sac of developing embryos. Cancer cell vascular invasion and extravasation is assessed via fluorescence microscopy of the tail region 24 to 96 hr later.
03:52
Experimental Metastatic Melanoma Mouse Model: A Platform to Study the Metastatic Potential of Melanoma Cells in the Lungs
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2023
In this video, melanoma cells are intravenously injected through the tail vein in the mouse model. The melanoma cells further travel through the circulatory system, adapting to the distant microenvironment and causing metastasis in organs like lungs.
FAQs
The sequence creates a controlled transition from blood flow to tissue entry. Endothelial cells first capture circulating white blood cells, allowing them to roll along the vessel wall. Adhesion then stabilizes their contact, after which the cells cross the endothelial barrier. This ordered progression helps immune cells leave circulation at sites where infection or injury requires a response.
Adhesion molecules enable the changing interactions between leukocytes and endothelial cells. They help capture circulating cells and support the transition from rolling to firm attachment against the vessel wall. Without this coordinated adhesion step, white blood cells would not be efficiently positioned to cross the endothelial barrier and reach affected surrounding tissues.
After adhering to the vessel wall, leukocytes cross the endothelial barrier by squeezing between endothelial cells or passing through them. These routes allow the cells to move out of the bloodstream while traversing the vascular lining. The crossing step is essential because it connects vascular capture with the arrival of immune cells in surrounding tissue.
Studying extravasation shows how blood vessels regulate access to tissues during immune responses. It connects endothelial behavior, leukocyte movement, and the organization of inflammation into a single biological process. This perspective helps researchers examine how vascular barriers support immune defense and how changes in cell movement may contribute to tissue damage or disease progression.
Extravasation can support cancer cell dissemination when tumor cells leave the bloodstream and enter surrounding tissues. The same general vascular transition that allows cells to move beyond blood vessels can therefore be relevant to metastasis. Examining this process helps connect vascular biology with the spread of cancer cells from circulation into new tissue environments.
Extravasation is also important when substances from the bloodstream leak into surrounding tissues, including situations involving drugs. Such leakage can produce complications because the substance reaches tissue outside the intended vascular space. Studying the process helps relate blood vessel behavior to tissue damage and provides biological context for understanding how vascular barriers can fail or be breached.