Female Mud Crabs

Female mud crabs are adult female members of the genus Scylla, ecologically and economically important crustaceans that support estuarine food webs, fisheries, and aquaculture. Their reproductive cycle involves ovarian development, mating, and storage of sperm in specialized spermathecae; when conditions promote spawning, mature eggs pass from the ovaries and are fertilized as they are released and attached beneath the abdomen. Studying female mud crabs helps researchers assess sexual maturation, fecundity, larval production, and population health. These findings guide hatchery practices, broodstock management, sustainable fisheries, and conservation of mangrove and estuarine habitats.

Female Mud Crabs - Related Videos

Research

JoVE Journal - Biology

Eyestalk Ablation to Increase Ovarian Maturation in Mud Crabs

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Cited by 5 •

2023

Two eyestalk ablation protocols (i.e., cauterization and surgery approaches) were performed on anesthetized female crabs. The eyestalk ablation of mud crabs hastened the ripening of ovaries without decreasing the survival rate.

Using the Horseshoe Crab, Limulus Polyphemus, in Vision Research

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Cited by 14 •

2009

In this video we perform electroretinogram recording, optic nerve recording, and intraretinal recording with the American horseshoe crab, Limulus Polyphemus. These electrophysiological paradigms can be used for investigating the neural basis of vision in a research or teaching lab.

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

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Cited by 27 •

2008

The American horseshoe crab, Limulus polyphemus, is arguably the most convenient source for large quantities of blood of any invertebrate. The blood is simple in composition, with only one cell-type in the general circulation, the granular amebocyte, and only three abundant proteins in the plasma, hemocyanin, the C-reactive proteins, and α2-macroglobulin. Blood is collected from the heart and the blood cells and plasma are separated by centrifugation.

Research

JoVE Journal - Neuroscience
Free Sample

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

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Cited by 4 •

2011

Here we present the methodology for fast and high resolution fluorescent voltage-sensitive dye imaging of detailed activity of neurons in the crab stomatogastric ganglion.

Obtaining Eggs from Xenopus laevis Females

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Cited by 10 •

2008

The eggs of Xenopus laevis intact, lysed, and/or fractionated are useful for a wide variety of experiments. This protocol shows how to induce egg laying, collect and dejelly the eggs, and sort the eggs to remove any damaged eggs.

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