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Blood Collection from the American Horseshoe Crab, Limulus Polyphemus
 

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

Article doi: 10.3791/958
October 13th, 2008 Usage Statistics

Summary October 13th, 2008

The American horseshoe crab, Limulus polyphemus, is arguably the most convenient source for large quantities of blood of any invertebrate. The blood is simple in composition, with only one cell-type in the general circulation, the granular amebocyte, and only three abundant proteins in the plasma, hemocyanin, the C-reactive proteins, and α2-macroglobulin. Blood is collected from the heart and the blood cells and plasma are separated by centrifugation.

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