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Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

Blood Collection from the American Horseshoe Crab, Limulus Polyphemus

Article DOI: 10.3791/958-v 12:49 min October 13th, 2008
October 13th, 2008



The American horseshoe crab, Limulus polyphemus, is arguably the most convenient source for large quantities of blood of any invertebrate. The blood is simple in composition, with only one cell-type in the general circulation, the granular amebocyte, and only three abundant proteins in the plasma, hemocyanin, the C-reactive proteins, and α2-macroglobulin. Blood is collected from the heart and the blood cells and plasma are separated by centrifugation.


Blood Collection Horseshoe Crab Limulus Polyphemus Immune System Blood Volume Granular Amebocyte Plasma Proteins Hemocyanin C-reactive Proteins α2-macroglobulin Cardiac Puncture Lipopolysaccharide Contamination Centrifugation Plasma Fractionation Blood Cells Microscopic Examination
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