Mechanical forces play a key role in lung development and lung injury. Here, we describe a method to isolate rodent fetal lung type II epithelial cells and fibroblasts and to expose them to mechanical stimulation using an in vitro system.
Wang, Y., Huang, Z., Nayak, P. S., Sanchez-Esteban, J. An Experimental System to Study Mechanotransduction in Fetal Lung Cells. J. Vis. Exp. (60), e3543, doi:10.3791/3543 (2012).