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An Experimental System to Study Mechanotransduction in Fetal Lung Cells
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JoVE Journal Bioengineering
An Experimental System to Study Mechanotransduction in Fetal Lung Cells

An Experimental System to Study Mechanotransduction in Fetal Lung Cells

DOI:
10.3791/3543-v

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09:35 min

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February 16, 2012

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, , ,
1Women & Infants Hospital of Rhode Island,Alpert Medical School of Brown University

Chapters

  • 00:05Title
  • 00:58Coating Culture Plates with Extra-cellular Matrix Proteins
  • 02:15Isolation of Fetal Rodent Lung Type II Epithelial Cells
  • 05:32Isolation of Fetal Rodent Lung Fibroblasts
  • 06:15Experimental System to Provided Mechanical Stimulation to Lung Cells
  • 07:42Representative Results from Mechanotransduction Experiments Using Fetal Lung Cells
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

Mechanical forces play a key role in lung development and lung injury. Here, we describe a method to isolate rodent fetal lung type II epithelial cells and fibroblasts and to expose them to mechanical stimulation using an in vitro system.

Tags

Experimental SystemMechanotransductionFetal Lung CellsMechanical ForcesLung DevelopmentAlveolar Type II Epithelial CellsPulmonary SurfactantFluid HomeostasisDistal Lung Parenchyma CellsExaggerated StretchMechanical VentilationMolecular MechanismsCellular MechanismsMechanical StimuliLung InjuryIsolation MethodFlexcell Strain UnitIn Vitro SystemMechanical Stimulation

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