Fibroblast Secreted Factor
Fibroblast Secreted Factor - Related Videos
08:45
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
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Cited by 9 •
2016
Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.
11:00
Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
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Cited by 1 •
2016
The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.
11:33
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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Cited by 2 •
2013
This article illustrates the use of pulse-chase radio labeling in combination with site-specific photocrosslinking to monitor interactions between a protein of interest and other factors in E. coli. Unlike traditional chemical cross-linking methods, this approach generates high resolution “snapshots” of an ordered assembly pathway in a living cell.
09:29
Assessing Cardiomyocyte Subtypes Following Transcription Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts
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Cited by 5 •
2017
This manuscript describes a step-by-step protocol for the generation and quantification of diverse reprogrammed cardiac subtypes using a retrovirus-mediated delivery of Gata4, Hand2, Mef2c, and Tbx5.
09:03
Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells
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Cited by 7 •
2014
We describe a matrigel plug assay to illustrate angiogenic potential of a pool of factors secreted by cancer cells using two complementary imaging modalities, ultrasound and endomicroscopy. The matrigel, an extracellular matrix (ECM)-mimic gel, is utilized to introduce the host (mouse) with angiogenic factors secreted to the conditioned media...
FAQs
Their effects arise through two complementary routes: receptor binding generates signals in neighboring cells, while interactions with the extracellular matrix alter the local tissue environment. These signals can change cell migration, proliferation, and tissue remodeling. Because multiple factor classes may act together, their combined activity helps coordinate cellular behavior within a tissue.
The major classes contribute different forms of tissue communication. Cytokines and chemokines are associated with immune and cellular signaling, growth factors influence growth-related tissue behavior, and extracellular matrix components affect the surrounding structural context. Their distinct roles give researchers a way to relate molecular signals to changes in migration, proliferation, or remodeling.
Abnormal secretion can disturb the coordinated signaling that supports tissue behavior. This dysregulation is linked to fibrosis and tumor progression, where tissue remodeling becomes pathological rather than appropriately controlled. Examining the released factors therefore helps connect altered fibroblast activity with disease mechanisms and identifies signaling changes that may be relevant to therapeutic research.
Wound healing provides a clear context because fibroblast-derived signals can coordinate cell migration, proliferation, and tissue remodeling during repair. Immune responses offer another, since the same signaling environment can influence communication among local cells. Studying both settings helps clarify how fibroblast activity contributes to tissue maintenance and how altered communication may affect tissue behavior.
Because these factors influence the tissue microenvironment, they provide biological signals that can be considered when evaluating biomaterials. Their effects on cell communication, migration, proliferation, and remodeling make them relevant to materials intended to interact with tissues. This connection allows biomaterials research to address not only structure, but also the signaling environment associated with tissue repair.
Research can ask whether tissue repair or pathological remodeling might be modified by changing these signals. The therapeutic relevance follows from their roles in wound healing, immune responses, fibrosis, and tumor progression. Understanding how factor release shapes local tissue behavior may support strategies aimed at promoting useful repair while limiting disease-associated remodeling.