Immunology and Infection
A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
Summary December 17th, 2013
This article illustrates the use of pulse-chase radio labeling in combination with site-specific photocrosslinking to monitor interactions between a protein of interest and other factors in E. coli. Unlike traditional chemical cross-linking methods, this approach generates high resolution “snapshots” of an ordered assembly pathway in a living cell.