Flexible Bone Experiment
Flexible Bone Experiment - Related Videos
07:28
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
0 Views •
Cited by 242 •
2013
We describe a protocol for isolation and purification of neutrophils from mouse bone marrow by density gradient centrifugation and for neutrophil labeling using CellTracker dyes. This represents a simple, fast, reproducible and economical method for obtaining large numbers of neutrophils for downstream functional studies or adoptive transfer and tracking experiments.
08:49
Models of Bone Metastasis
0 Views •
Cited by 115 •
2012
Animal models are frequently utilized to study cancer metastasis to bone. In this protocol we will describe two common methods of tumor inoculation for bone metastasis studies and briefly describe some of the analyses utilized to monitor and quantify these models.
08:30
Operant Procedures for Assessing Behavioral Flexibility in Rats
0 Views •
Cited by 69 •
2015
The ability to assess executive functions such as behavioral flexibility in rats is useful for investigating the neurobiology of cognition in both intact animals and disease models. Here we describe automated tasks for assessing strategy shifting and reversal learning, which are particularly sensitive to disruptions in prefrontal cortical networks.
03:47
Implantation of a Flexible Biocompatible Probe in a Glioblastoma Mouse Model
0 Views •
2025
Source: Lefevre, M. C., et al., Flexible Organic Electronic Devices for Pulsed Electric Field Therapy of Glioblastoma. J. Vis. Exp. (2022)This video demonstrates the implantation of a flexible, biocompatible probe in a glioblastoma mouse model to deliver targeted pulsed electric field therapy for neurotherapeutic applications.
07:17
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
0 Views •
Cited by 293 •
2016
Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.
FAQs
The acidic solution dissolves much of the bone’s mineral content, especially calcium salts, while leaving the collagen-rich organic framework relatively intact. Removing mineral reduces hardness, whereas the remaining collagen framework allows greater flexibility. The resulting change provides a visible demonstration that bone mechanics depend on the interaction between inorganic mineral components and organic tissue.
Calcium salts contribute substantially to bone hardness, while collagen provides an organic framework associated with flexibility. The experiment changes the balance between these components by reducing mineral content without removing most of the collagen framework. Comparing the treated and untreated specimens helps connect microscopic composition with observable mechanical properties.
An untreated bone provides a comparison point for the acid-treated specimen. If the treated bone bends more easily and feels softer, those observations link the loss of mineral content to reduced hardness. This comparison supports a central biology principle: tissue composition influences function, rather than structure being mechanically uniform throughout.
Students can compare an animal bone before and after soaking it in an acidic solution, focusing on hardness and bendability. The untreated specimen serves as a reference, while the treated specimen reveals the effect of mineral removal. Recording visible and mechanical differences turns the activity into a direct investigation of bone composition.
The standard setup requires an animal bone, an acidic solution, and an untreated bone or initial observation for comparison. The acid-treated sample demonstrates what happens when much of the calcium-rich mineral component is dissolved, while the untreated sample represents the original mechanical condition. Together, they provide the basis for interpreting the change.
This investigation gives students a practical way to connect tissue composition with mechanical function. Rather than studying mineralization and collagen only as abstract terms, they can observe how altering bone composition changes hardness and flexibility. It also introduces experimental observation by linking a treatment, a comparison specimen, and a measurable physical outcome.