Fluorescent Oxygen Sensor
Fluorescent Oxygen Sensor - Related Videos
09:14
Glovebox and Impurity Sensors
0 Views •
2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University The glovebox provides a straightforward means to handle air- and moisture-sensitive solids and liquids. The glovebox is what it sounds like: a box with gloves attached to one or more sides, which allows the user to perform manipulations within the glovebox under an inert atmosphere. For manipulations under inert atmospheres, chemists can choose between Schlenk or high-vacuum techniques and a glovebox. Schlenk and...
09:57
Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors
0 Views •
Cited by 5 •
2016
A method for imaging changes in membrane potential using genetically encoded voltage indicators is described.
11:56
Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
0 Views •
Cited by 1 •
2010
Fabrication and validation of an add-on platform that offers enhanced control over the spatial and temporal oxygenation in a 6-well plate. The device is adaptable to a number of culture systems and can be used to investigate the effects of oxygen on wound healing.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
0 Views •
Cited by 27 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
09:08
Detecting Reactive Oxygen Species
0 Views •
2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
FAQs
Molecular oxygen acts as a dynamic quencher of the excited indicator. After illumination, oxygen molecules collide with the fluorophore and reduce its emitted fluorescence. The size of that change carries information about oxygen availability, linking an optical signal to conditions in a biological or chemical system. This mechanism makes oxygen detectable without relying on a visible change in the sample.
Calibration converts measured fluorescence changes or lifetimes into oxygen estimates. It establishes how the indicator’s optical response corresponds to known oxygen levels, while the selected readout can be intensity or lifetime. This step is important for interpreting measurements consistently across biological cultures, tissues, or chemical systems.
Fluorescence lifetime provides a time-based alternative to measuring emission intensity. Because oxygen can quench the excited indicator, changes in how long fluorescence persists can report oxygen levels when calibrated. Using lifetime or intensity gives the measurement a defined optical basis for assessing oxygen availability in the sample.
A basic measurement sequence begins by illuminating the sensor’s indicator, then recording its fluorescence response. The measured intensity or lifetime is compared with a calibration relating optical behavior to oxygen level. This workflow can be applied to dissolved oxygen or localized regions in cultures and organisms, allowing investigators to connect the readout with biological or chemical conditions.
Within biology, these sensors can support studies of cellular respiration, tissue oxygenation, and hypoxic microenvironments. Measurements may be made in cultures or organisms, so oxygen data can be considered alongside physiological or metabolic conditions. The same analytical approach also applies to dissolved oxygen and environmental effects on living systems, extending its use beyond a single experimental scale.
Optical readout is especially useful when researchers need oxygen information from a defined location without invasive measurements. Fluorescent measurements can therefore examine localized oxygen availability in tissues, cultures, or organisms while preserving the connection between the signal and the sampled microenvironment. This spatial capability supports studies of physiology, metabolism, and hypoxia at scales relevant to living systems.