Fluorimeter Detection
Fluorimeter Detection - Related Videos
09:00
Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Cited by 15 •
2009
The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.
13:20
Detection of Bacteria Using Fluorogenic DNAzymes
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Cited by 17 •
2012
We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental...
06:47
Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4
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2025
In this protocol, a combined recombinase polymerase amplification CRISPR/Cas12a assay is illustrated for the detection of the invasive plant pathogen, Fusarium oxysporum f. sp. cubense tropical race 4.
09:36
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
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Cited by 5 •
2021
Detailed instructions are provided to build an open-source, modular fluorimeter that is compatible with many low-cost heaters to perform real-time, quantitative isothermal nucleic acid amplification.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
FAQs
The measurement separates the light used to excite a sample from the fluorescence produced afterward. Emitted light appears at a longer wavelength than the excitation light, allowing the instrument to distinguish the signal from the illumination beam. This separation is essential for detecting fluorescent molecules accurately rather than confusing emitted fluorescence with the original excitation light.
Under controlled conditions, the measured emission intensity provides a basis for relating signal strength to the amount of fluorescent molecule present. Greater or lesser intensity can therefore support quantitative analysis, provided the measurement conditions remain consistent. This principle allows Fluorimeter Detection to move beyond identifying a fluorophore and provide concentration-related information for biological samples.
Optical filters or a monochromator select the relevant wavelengths during measurement. They help separate emitted fluorescence from the excitation beam, improving the instrument’s ability to isolate the signal of interest. This optical selection supports the high sensitivity of fluorimeter measurements and makes the resulting fluorescence data more useful for detecting or quantifying molecules.
A typical measurement begins with placing a biological sample in the fluorimeter and illuminating it at a selected excitation wavelength. The instrument then collects light emitted at a longer wavelength while using optical separation to distinguish it from the excitation beam. The resulting emission intensity can be interpreted for identification or quantitative analysis under controlled conditions.
The method supports a broad range of biological targets, including nucleic acids, proteins, enzyme activity, and cellular processes. The specific target determines what the fluorescence measurement represents, such as the presence or amount of a molecule or a change associated with biological activity. This flexibility makes the technique relevant across molecular and cellular biology.
Fluorimeter Detection is useful when researchers need quantitative information from fluorescent biological samples. Applications include research assays, diagnostic measurements, monitoring enzyme activity or cellular processes, and studying molecular interactions. Because fluorescence can provide sensitive measurements, the approach helps connect optical signals with biological questions involving molecule detection, concentration, activity, or interaction.