Fluorometry Capability
Fluorometry Capability - Related Videos
19:26
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
0 Views •
Cited by 30 •
2013
Intracellular Ca2+ dynamics are very important in sperm physiology and Ca2+-sensitive fluorescent dyes constitute a versatile tool to study them. Population experiments (fluorometry and stopped flow fluorometry) and single cell experiments (flow cytometry and single cell imaging) are used to track spatio-temporal [Ca2+] changes in human sperm cells.
09:24
Voltage-clamp Fluorometry in Xenopus Oocytes Using Fluorescent Unnatural Amino Acids
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Cited by 13 •
2017
This article describes an enhancement of conventional Voltage-Clamp Fluorometry (VCF) where Fluorescent Unnatural Amino Acids (fUAA) are used instead of maleimide dyes, to probe structural rearrangements in ion channels. The procedure includes Xenopus oocyte DNA injection, RNA/fUAA coinjection, and simultaneous current and fluorescence measurements.
09:12
Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
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Cited by 4 •
2013
The system described herein employs a traditional optical trap as well as an independent holographic optical trapping line, capable of creating and manipulating multiple traps. This allows for the creation of complex geometric arrangements of refractive particles while also permitting simultaneous high-speed, high-resolution measurements of the activity of biological enzymes.
05:27
An In Vitro Assay to Study the Cytotoxic Capability of Cytokine-Induced Killer Cells
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2025
This video demonstrates an in vitro technique to measure the cytotoxic ability of cytokine-induced killer (CIK) cells against cancer cells. Upon co-culturing target cancer cells with CIK cells, the dead cancer cells are identified using a fluorescent viability dye and are quantified using flow cytometry, estimating the cytotoxic effect of CIK cells.
09:09
Synthesis of Poly(N-isopropylacrylamide) Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
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Cited by 3 •
2016
We present a protocol to synthesize Janus microhydrogels composed entirely of the same base material, poly(N-isopropylacrylamide) (PNIPAAm), with a clearly compartmentalized structure base on the phase separation of a supersaturated NIPAAm monomer solution. The synthesized Janus microhydrogels show unique properties such as anisotropic thermo-responsiveness and organophilic/hydrophilic loading capability.
FAQs
Fluorometry separates the excitation signal from fluorescence by using a selected excitation wavelength and collecting emitted light at a longer wavelength. Optical filters help transmit the relevant emitted signal while limiting unwanted wavelengths, and the detector converts that collected light into a measurement. This spectral separation allows the instrument to associate the signal with the fluorescent substance being analyzed.
Positioning the detector at a right angle to the excitation path reduces direct interference from the incoming excitation beam. That geometry helps the instrument measure emitted fluorescence rather than simply detecting excitation light through the sample. In practice, this optical arrangement supports cleaner readings, which is important when the fluorescent signal is weak or the target is present at low abundance.
Fluorometry capability is useful for concentration measurements because the observed fluorescent signal provides information about how much fluorescent substance is present. Its sensitivity also supports detection of low-abundance targets. Interpretation depends on selecting an excitation wavelength that addresses the fluorophore and collecting the appropriate longer-wavelength emission, so optical settings are central to obtaining meaningful quantification.
A typical measurement begins by selecting an excitation wavelength for the fluorophore. The sample is illuminated, and emitted light is collected separately through optical filters, commonly with detection at a right angle to the excitation beam. The detector then records the fluorescence signal for assessing target presence or concentration.
In biology, the approach can support measurements of nucleic acids, proteins, and metabolites, allowing researchers to examine different classes of biological molecules with one fluorescence-based analytical capability. The same general platform can therefore serve molecular assays across varied sample types, provided the target produces or carries a measurable fluorescent signal.
Fluorescently labeled cells or molecules create measurable signals that fluorometry can monitor, extending the method beyond static detection of isolated substances. Because fluorescence measurements can monitor dynamic biological processes, the capability is relevant when researchers need information about changes over time or the behavior of labeled biological targets within an experiment.
It is especially valuable when a study or diagnostic measurement requires sensitivity for low-abundance targets. Biological researchers can apply it to nucleic acids, proteins, metabolites, or fluorescently labeled cells and molecules, while diagnostic settings can use the same capacity to obtain information about target presence and concentration.