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JoVE Journal
Engineering

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Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
 

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Article DOI: 10.3791/50481 09:12 min
April 22nd, 2013

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Summary April 22nd, 2013

The system described herein employs a traditional optical trap as well as an independent holographic optical trapping line, capable of creating and manipulating multiple traps. This allows for the creation of complex geometric arrangements of refractive particles while also permitting simultaneous high-speed, high-resolution measurements of the activity of biological enzymes.

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