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Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
JoVE Journal
Engineering
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JoVE Journal Engineering
Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
DOI:

09:12 min

April 22, 2013

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Chapters

  • 00:05Title
  • 00:54Installation of 980 nm Wavelength Single Optical Trap
  • 04:08Installation of 1,064 nm Wavelength Holographic Trap
  • 05:35System Installation and Alignment Notes
  • 07:24Results: Trapping Multiple Refractive Objects
  • 08:42Conclusion

Summary

Automatic Translation

The system described herein employs a traditional optical trap as well as an independent holographic optical trapping line, capable of creating and manipulating multiple traps. This allows for the creation of complex geometric arrangements of refractive particles while also permitting simultaneous high-speed, high-resolution measurements of the activity of biological enzymes.

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