Green Nanoparticles
Green Nanoparticles - Related Videos
08:51
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
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Cited by 9 •
2013
A protocol for nanoparticle tracking analysis (NTA) and high-throughput flow cytometry to evaluate polymeric gene delivery nanoparticles is described. NTA is utilized to characterize the nanoparticle particle size distribution and the plasmid per particle distribution. High-throughput flow cytometry enables quantitative transfection efficacy evaluation for a library of gene delivery biomaterials.
12:47
Tangential Flow Ultrafiltration: A “Green” Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles
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Cited by 20 •
2012
Tangential flow ultrafiltration (TFU) is a recirculation method used for the weight-based separation of biosamples. TFU was adapted to size-select (1-20 nm diameter) and highly concentrate a large volume of polydisperse silver nanoparticles (4 L of 15.2 μg ml-1 down to 4 ml of 8,539.9 μg ml-1) with minimal aggregation.
10:51
Protocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy
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Cited by 11 •
2013
We describe the protocols used to investigate the interactions of 13.56 MHz radiofrequency (RF) electric-fields with gold nanoparticle colloids in both non-biological and biological systems (in vitro/vivo). These interactions are being investigated for applications in cancer therapy.
09:23
Harmonic Nanoparticles for Regenerative Research
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Cited by 1 •
2014
Protocol details are provided for in vitro labeling human embryonic stem cells with second harmonic generating nanoparticles. Methodologies for hESC investigation by multi-photon microscopy and their differentiation into cardiac clusters are also presented.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
FAQs
During synthesis, plant extracts, microorganisms, or isolated biomolecules supply phytochemicals, enzymes, and other reducing agents. These substances convert metal ions into nanoscale particles rather than requiring only externally supplied chemical reagents. The same biological components can also remain associated with the particle surface, creating a coating that helps stabilize the developing material and affects its later biological interactions.
Size, shape, composition, and surface coating are key variables because they determine how particles behave and interact with cells. Changes in these properties can alter reactivity and the way biological systems respond to the material. Consequently, green nanoparticle studies must characterize more than composition alone when evaluating suitability for antimicrobial, sensing, imaging, or delivery applications.
The biological coating has a functional role in addition to stabilizing the nanoparticle surface. Because it originates from phytochemicals, enzymes, or other biomolecules used during synthesis, it can influence how the material interacts with cells. This connection between synthesis inputs and surface behavior helps explain why biologically produced particles may show different responses from materials with other surface characteristics.
A typical workflow begins by selecting a biological source, such as a plant extract, microorganism, or biomolecule preparation, and bringing it into contact with metal ions. Reducing agents then convert the ions into nanoparticles while biological components simultaneously coat and stabilize the surfaces. Researchers can subsequently examine properties such as size, shape, composition, and biological coating.
They are useful when a study requires nanoscale materials for antimicrobial research, biosensing, imaging, or targeted delivery. Their biological coating and tunable size, shape, and composition can affect interactions with cells, making characterization central to interpreting results. These applications place green nanoparticles within nanobiology as materials for investigating both biological detection and material-cell interactions.
Green nanoparticles connect biological resources and processes with broader sustainable nanomaterial development. Plant extracts, microorganisms, and biomolecules provide synthesis-related functions, while the resulting materials can support environmental applications in addition to biological research. This combination makes the topic relevant to studies seeking nanomaterials produced with less reliance on hazardous chemicals and energy-intensive processing.