Fibroblastic Foci
Fibroblastic Foci - Related Videos
04:09
Assessing DNA Damage Foci: To Identify Radiation-Induced DNA Damage Foci in Cancer Cell Line Using Immunofluorescence Staining
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2023
This video describes a protocol to detect radiation-induced DNA repair foci in the colon cancer cell lines using immunofluorescence staining. DNA repair proteins get activated at the DNA damage site; therefore, we can detect them using specific antibodies and visualize them under a fluorescence microscope.
08:48
Quantitation of γH2AX Foci in Tissue Samples
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Cited by 7 •
2010
Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.
06:53
Quantification of γH2AX Foci in Response to Ionising Radiation
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Cited by 23 •
2010
Quantification of DNA double-strand streaks using γH2AX formation as a molecular marker has become an invaluable tool in radiation biology. Here we demonstrate the use of an immunofluorescence assay for quantification of γH2AX foci after exposure of cells to radiation.
09:33
Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules
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Cited by 33 •
2017
Stress Granules (SGs) are nonmembranous cytoplasmic structures that form in cells exposed to a variety of stresses. SGs contain mRNAs, RNA-binding proteins, small ribosomal subunits, translation-related factors, and various cell signaling proteins. This protocol describes a workflow that uses several experimental approaches to detect, characterize, and quantify bona fide SGs.
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
FAQs
After epithelial or tissue injury, damaged cells and inflammatory mediators provide signals that first promote fibroblast migration and proliferation. Some of these fibroblasts then differentiate into myofibroblasts as the focus develops within remodeling tissue. This sequence connects the initial injury response to extracellular-matrix accumulation and helps explain how persistent repair can become fibrotic rather than restorative.
Myofibroblast differentiation adds a contractile, matrix-producing cell population to the injury response. These cells contribute to deposition of collagen-rich extracellular matrix within the affected tissue, extending the remodeling process beyond initial fibroblast accumulation. In pulmonary tissue, that activity helps create microscopic changes that researchers can relate to the progression of abnormal wound healing.
Collagen-rich extracellular matrix shows that repair has progressed from cellular recruitment toward structural remodeling. Its accumulation provides a microscopic link between activated fibroblasts, myofibroblasts, and changes in tissue architecture. In lung studies, examining this remodeling helps researchers connect local fibrotic lesions with impaired tissue function rather than viewing the cellular aggregates in isolation.
Location and apparent activity provide context for interpreting these lesions within damaged tissue. In pulmonary biology, fibroblastic foci commonly occur along injured alveolar walls, where their distribution can be considered alongside the surrounding remodeling. Evaluating both features helps pathologists and researchers relate microscopic patterns to the extent of lung fibrosis and abnormal repair.
A microscopic assessment should consider the presence, distribution, and activity of the cellular aggregates, especially in relation to damaged alveolar walls. Researchers can then compare these observations with the surrounding collagen-rich remodeling and the broader pattern of lung fibrosis. This approach supports interpretation of how localized lesions contribute to tissue-level structural and functional changes.
Their occurrence along damaged alveolar walls is characteristic of usual interstitial pneumonia, including idiopathic pulmonary fibrosis. Consequently, identifying and evaluating these lesions gives pathologists a histologic feature for relating cellular repair activity to pulmonary fibrosis. The findings also help connect the microscopic appearance of lung tissue with remodeling that may impair normal tissue function.
Because fibroblastic foci reflect active cellular remodeling and collagen-rich matrix deposition, their distribution and activity can provide tissue-level information when potential antifibrotic treatments are evaluated. Researchers can examine whether treatment studies show relevant changes in these microscopic features and then relate those observations to the broader pattern of lung fibrosis and tissue impairment.