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The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
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Chapters
- 00:05Title
- 00:49Collection and Preparation of the Colon
- 02:30Colon Organ Culture
- 04:43E. coli Killing Assay
- 06:26Effect of Extrinsic and Intrinsic Factors on Colonic Antimicrobial Host Defense Responses
- 08:51Results: Intestinal Antimicrobial Immune Responses in Ex Vivo Cultured Colon
- 10:11Conclusion
The ex vivo organ culture allows investigation of biological processes in the context of the intact tissue architecture. Here, we introduce a method of ex vivo culture of the mouse colon, which can be used to study innate immunity and antimicrobial host defense in the intestine.
Tags
Keywords:
Ex VivoColon Organ CultureAntimicrobial Host DefensePathogen Associated Molecular PatternsPattern Recognition ReceptorsInnate Immune PathwaysAntimicrobial PeptidesIntestinal ArchitectureEuthanasiaPeritoneumCecumRectumPBSDMEM F12FBSPenicillin StreptomycinGentamycinCell Strainer12-well Cell Culture Plate
