Hybridization Detection
Hybridization Detection - Related Videos
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
03:26
Probe Hybridization and Signal Amplification in RNA In Situ Hybridization: A Technique for Detecting Specific RNA Sequences in Tissue Sections
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2023
This video describes a sequential hybridization strategy that involves hybridizing mRNA probes to specific target mRNA. Following probe hybridization, signal amplification is performed to enhance resolution via reduction of signal-to-noise ratio during RNA-CISH of histological samples.
09:16
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
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Cited by 7 •
2015
Array CGH for the detection of genomic copy number variants has replaced G-banded karyotype analysis. This paper describes the technology and its application in a diagnostic service laboratory.
11:24
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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Cited by 8 •
2015
RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.
FAQs
Temperature and salt concentration are controlled to regulate how selectively the probe pairs with nucleic acid sequences. Together with sequence complementarity, these conditions influence whether binding produces a sufficiently specific duplex rather than an ambiguous signal. Researchers therefore adjust the hybridization environment to distinguish closely related sequences and improve confidence that the detected signal corresponds to the intended target.
Sequence complementarity determines how closely the labeled probe matches a target strand. A sequence variation can alter that matching relationship and consequently affect duplex formation or the resulting measurable signal. By comparing detection patterns for related sequences, researchers can use hybridization detection to investigate genetic variation and identify changes associated with mutation analysis.
The probe provides the link between molecular recognition and observation. After it pairs with a matching target, its label enables the resulting duplex to generate a measurable signal. This feature allows researchers to determine whether a particular nucleic acid sequence is present in a sample, rather than relying only on an unobservable pairing event.
A useful experiment requires control of the environment in which probe and target strands interact, especially temperature and salt concentration. Sequence complementarity must also be considered because it affects binding specificity. Managing these variables helps researchers interpret the measured signal as evidence for a particular target sequence and supports more reliable comparisons among samples.
Researchers select a probe designed to correspond to the sequence of interest and examine whether a detectable signal appears after interaction with the sample. The result indicates whether that sequence is present, allowing gene identification and broader characterization of nucleic acids. This approach is especially useful when the research question concerns a specific genetic sequence rather than the entire sample.
The technique supports several biology applications, including pathogen detection, mutation analysis, gene identification, and measurement of gene expression. In each case, the signal provides evidence about a selected nucleic acid sequence in the sample. These results help researchers examine genetic variation, diagnose biological conditions, and investigate how genetic information is represented or expressed.