Journal
/
Neuroscience
/
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

DOI:
10.3791/52799-v

•

11:24 min

•

June 17, 2015

•

, , ,
1College of Physicians and Surgeons,Columbia University

Chapters

  • 00:05Title
  • 01:26Heat-Induced Antigen/RNA Unmasking
  • 02:21Probe Hybridization
  • 04:01Immunofluorescence Staining
  • 05:42Diaminobenzidine (DAB)-based Chromogenic ISH Assay Followed by Luxol Fast Blue and Cresyl Violet Stain Using Heat-induced and Protease-induced RNA Unmasking
  • 08:54Results: Aft4 Localization to Cholinergic Axons in the Mouse Dentate Gyrus and Myelinated Axons in the Adult Hippocampal Formation
  • 10:53Conclusion

Summary

Automatic Translation

Automatic Translation

RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.

Tags

Keywords: Axonally Localized MRNAsIn Situ HybridizationRNAscopeAxonal CounterstainFluorescence ImmunohistochemistryHistological DyesAtf4 MRNATranscriptome AnalysisVertebrate AxonsAxon PathfindingAxon BranchingNeurodegenerationSingle-molecule DetectionSubcellular LocalizationMature Mouse BrainHuman Brain

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52799?article_type=v

Double Fluorescence in situ Hybridization in Fresh Brain Sections
check_url/52799?article_type=v

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology
check_url/52799?article_type=v

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
check_url/52799?article_type=v

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
check_url/52799?article_type=v

Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey
check_url/52799?article_type=v

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging
check_url/52799?article_type=v

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
check_url/52799?article_type=v

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
check_url/52799?article_type=v

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology
check_url/52799?article_type=v

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

Read Article