Hydronium Ion Concentration

Hydronium ion concentration is the amount of H₃O⁺ present in an aqueous solution, providing a direct measure of its acidity and a foundation for understanding pH. It arises when water molecules accept protons, and pH expresses this concentration logarithmically as −log₁₀[H₃O⁺], so small changes can represent substantial shifts in acidity. In biology, hydronium levels influence protein structure, enzyme activity, membrane transport, and cellular metabolism. Buffer systems, including weak acids and their conjugate bases, help stabilize these levels in blood, cytoplasm, and organelles, while measured changes can reveal physiological dysfunction or altered biochemical conditions.

Hydronium Ion Concentration - Related Videos

Research

JoVE Journal - Bioengineering

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

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2017

The protocol for a novel ion concentration polarization (ICP) platform that can stop the propagation of the ICP zone, regardless of the operating conditions is described. This unique ability of the platform lies in the use of merging ion depletion and enrichment, which are two polarities of the ICP phenomenon.

Education

JoVE Core - Chemistry

Common Ion Effect

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2020

Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Châtelier’s principle. Consider the dissolution of silver iodide: This solubility equilibrium may be shifted left by the addition of either silver(I) or iodide ions, resulting in...

Research

JoVE Journal - Bioengineering
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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

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Cited by 24 •

2011

Fluorescent nanoparticles produced in our lab are used for imaging ion concentrations and ion fluxes in biological systems such as cells during signaling and interstitial fluid during physiological homeostasis.

Measurement of Evoked Potassium Ion Concentration Dynamics in Coronal Hippocampal Slices

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2025

The video demonstrates evoked potassium ion concentration dynamics on electrical stimulation of mouse coronal hippocampal brain slices. A bipolar stimulating electrode is inserted into the stratum radiatum of the CA3 region in the hippocampus, and a potassium-ion selective electrode is placed in the CA1 stratum radiatum. The electrical stimulation causes an action potential, resulting in potassium ion release. Finally, the lack of evoked potassium ion response in the presence of an inhibitory...

Research

JoVE Journal - Neuroscience
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Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

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Cited by 18 •

2015

We demonstrate the fabrication, calibration and properties of two types of ion-selective microelectrodes (double-barreled and concentric) for measurement of ion concentrations in brain tissue. These are then used in the mouse hippocampal slice preparation to show that excitatory activity changes both extracellular potassium and sodium concentrations.

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