Induced Fit
Induced Fit - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
0 Views •
2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
04:50
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
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Cited by 10 •
2018
Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.
09:08
Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
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Cited by 1 •
2025
This study describes a method for high-throughput experiments using a 3D-printed LED array to optimize light-inducible gene expression in HEK293T cells.
FAQs
Binding does more than attach a ligand: it reshapes the enzyme’s active site so catalytic groups adopt productive positions. This rearrangement can also stabilize the transition state, the high-energy stage associated with conversion to product. Consequently, induced fit links molecular recognition to catalysis, explaining how binding can directly improve the chemical conditions for an enzyme reaction.
Induced fit differs from the lock-and-key model because it treats the binding site as flexible rather than rigid. A ligand can therefore promote a compatible conformation instead of simply occupying a preformed cavity. This flexibility helps explain selective interactions while allowing the protein to adjust its functional state as binding occurs.
In allosteric regulation, ligand binding can stabilize a particular protein conformation and thereby influence activity even when the binding event is not described as direct substrate conversion. The same conformational principle contributes to signal transduction, where a binding event is associated with a structural change that affects the protein’s functional state.
A useful conceptual workflow compares the protein before and after ligand binding, focusing on whether the active site changes shape and whether catalytic groups become positioned for reaction. This comparison connects structural rearrangement with transition-state stabilization and product formation, rather than treating binding as a static docking event.
Induced fit helps drug design account for protein flexibility rather than assuming that a target has one permanently fixed binding shape. A small molecule may stabilize a particular protein conformation and alter activity. This perspective supports the study of compounds that influence protein function by favoring specific structural states.
The model explains specificity by connecting selective ligand recognition with the structural changes required for catalysis. A compatible substrate can promote an active-site arrangement that positions catalytic groups and supports transition-state stabilization, whereas binding is not merely passive occupancy. This makes induced fit useful for relating molecular shape, enzyme activity, and product formation.