Infrared Detection
Infrared Detection - Related Videos
08:16
Infrared Thermography for the Detection of Changes in Brown Adipose Tissue Activity
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Cited by 6 •
2022
Here, we present a protocol for measuring brown adipose tissue activity after a meal in humans and laboratory animals.
08:11
Infrared Spectroscopy
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2023
Source: Vy M. Dong and Zhiwei Chen, Department of Chemistry, University of California, Irvine, CA This experiment will demonstrate the use of infrared (IR) spectroscopy (also known as vibrational spectroscopy) to elucidate the identity of an unknown compound by identifying the functional group(s) present. IR spectra will be obtained on an IR spectrometer using the attenuated total reflection (ATR) sampling technique with a neat sample of the unknown.
09:38
Characterizing Far-infrared Laser Emissions and the Measurement of Their Frequencies
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Cited by 4 •
2015
We describe the generation of far-infrared radiation using an optically pumped molecular laser along with the measurement of their frequencies with heterodyne techniques. The experimental system and techniques are demonstrated using difluoromethane (CH2F2) as the laser medium whose results include three new laser emissions and eight measured laser frequencies.
10:12
Near-infrared Fluorescence Imaging of Abdominal Aortic Aneurysms
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2023
Source: Arvin H. Soepriatna1, Kelsey A. Bullens2, and Craig J. Goergen1 1 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 2 Department of Biochemistry, Purdue University, West Lafayette, Indiana Near-infrared fluorescence (NIRF) imaging is an exciting optical technique that utilizes fluorescent probes to visualize complex biomolecular assemblies in tissues. NIRF imaging has many advantages over conventional imaging methods for noninvasive imaging of diseases.
10:42
Infrared Degenerate Four-wave Mixing with Upconversion Detection for Quantitative Gas Sensing
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2019
Here, we present a protocol to perform sensitive, spatially resolved gas spectroscopy in the mid-infrared region, using degenerate four-wave mixing combined with upconversion detection.
FAQs
Infrared detection can operate through two complementary signal pathways. Some sensors respond directly to infrared photons, whereas others detect the heat associated with incoming radiation and convert the incoming signal into electrical output. This conversion allows imaging and spectroscopy systems to quantify radiation and examine biological patterns across space or wavelength.
Temperature-related measurements are interpreted through infrared intensity patterns, while chemical information is examined by comparing how intensity varies across wavelength. Spectroscopy therefore adds a wavelength-resolved dimension that imaging alone may not provide. In biological studies, this distinction helps researchers select measurements suited to thermal state, molecular signatures, or both.
Spatial analysis shows where infrared signals occur across an organism, tissue, or cell, whereas wavelength analysis characterizes how those signals change across the infrared range. Using either dimension separately or together can connect a measured pattern with location, temperature, chemical composition, or biological activity, improving interpretation of physiological observations.
A typical workflow directs radiation from an organism or biological sample to a sensor, converts the response into electrical signals, and analyzes the resulting intensity pattern. Imaging examines variation across space, while spectroscopy examines variation across wavelength. Researchers can then relate the measurements to physiological changes or biological responses under controlled or natural conditions.
Thermal imaging is useful when researchers need to observe temperature-related patterns across an organism without relying on invasive sampling. It can support investigations of physiological changes and biological responses by showing how thermal signals vary across space. Studies may be conducted under controlled conditions or in natural settings, depending on the research question.
Infrared measurements can help researchers monitor blood flow and metabolism as noninvasive indicators of physiological activity. By recording radiation from biological systems and analyzing its spatial or wavelength-dependent intensity, investigators can track changes associated with these processes. The resulting measurements may help assess normal responses or identify abnormalities in tissues and organisms.
Infrared spectroscopy can examine molecular signatures in tissues and cells by analyzing radiation intensity across wavelength. These signatures provide information complementary to spatial thermal patterns, allowing researchers to investigate chemical characteristics alongside physiological state. Such measurements are relevant for assessing biological changes and supporting the identification of abnormalities without requiring invasive analysis.