Intercalated Cells
Intercalated Cells - Related Videos
11:02
Synthesis and Catalytic Performance of Gold Intercalated in the Walls of Mesoporous Silica
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2015
Here, we present a protocol with a sol-gel process to synthesize gold intercalated in the walls of mesoporous materials (GMS), which is confirmed to possess a mesoporous matrix with gold intercalated in the walls imparting great stability and recyclability.
06:40
Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
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Cited by 10 •
2015
Cell organization of craniofacial bones has long been hypothesized but never directly visualized. Multi-spectral cell labeling and in vivo live imaging allows visualization of dynamic cell behavior in zebrafish lower jaw. Here, we detail the protocol to manipulate Zebrabow transgenic fish and directly observe cell intercalation and morphological changes of chondrocytes in the Meckel’s cartilage.
09:32
Cell Cycle Analysis
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2023
Cell cycle refers to the set of events through which a cell grows, replicates its genome, and ultimately divides into two daughter cells through the process of mitosis. Because the amount of DNA in a cell shows characteristic changes throughout the cycle, techniques known as cell cycle analysis can be used to separate a population of cells according to the different phases of cell cycle they are in, based on their varying DNA content.This video will cover the principles behind cell cycle...
22:49
Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes
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Cited by 45 •
2012
Successful use of cell tracking dyes to monitor immune cell function and proliferation involves several critical steps. We describe methods for: 1) obtaining bright, uniform, reproducible label-ing with membrane dyes; 2) selecting fluorochromes and data acquisition conditions; and 3) choosing a model to quantify cell proliferation based on dye dilution.
02:40
Natural Killer Cell-Mediated Cytotoxicity Assay Sample Preparation for Flow Cytometric Analysis
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2025
This video demonstrates the lytic activity of natural killer cells against fluorescently-stained target cells. The method enables NK cell-induced cytotoxicity measurement by staining dead cells and using a flow cytometer to quantify live and dead target cells.
FAQs
Alpha-intercalated cells move hydrogen ions into the collecting-duct lumen using apical H+-ATPases. At the same time, they reabsorb bicarbonate, linking urinary hydrogen-ion secretion with preservation of this important base in the body. These coordinated transport activities promote urinary acidification and help correct conditions in which systemic acid levels are excessive.
Beta-intercalated cells provide a complementary transport pattern by secreting bicarbonate through the pendrin transporter. This activity supports bicarbonate removal in urine and helps retain hydrogen ions. Their function becomes especially relevant when acid-base conditions require the kidney to shift away from hydrogen-ion secretion and toward processes that reduce excess base.
The two cell types regulate acid-base chemistry in opposite directions. Alpha-intercalated cells favor hydrogen-ion secretion and bicarbonate reabsorption, whereas beta-intercalated cells favor bicarbonate secretion and hydrogen-ion retention. This division allows collecting ducts to adjust renal transport according to changing acid-base conditions rather than relying on one fixed response.
Transport by intercalated cells connects the composition of tubular fluid with systemic pH. Hydrogen-ion secretion increases urinary acidification, while bicarbonate handling determines whether base is conserved or excreted. By altering these processes in response to acid-base status, the cells help maintain blood pH and coordinate renal compensation for disturbances.
Studies commonly focus on how intercalated-cell organization and transport activity regulate acid-base balance in the kidney. Investigators can relate the behavior of alpha- and beta-cell pathways to hydrogen-ion secretion, bicarbonate handling, urinary acidification, and systemic pH control. This provides a cellular framework for understanding renal contributions to homeostasis.
Impaired intercalated-cell transport can disrupt the kidney’s ability to regulate hydrogen ions and bicarbonate. Research therefore examines these cells in metabolic acidosis, electrolyte disorders, and kidney diseases that interfere with acid-base regulation. Comparing transport activity and cellular organization helps clarify how renal dysfunction may contribute to abnormal systemic pH.