Metabolic Pathway
Metabolic Pathway - Related Videos
07:26
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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Cited by 53 •
2012
13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.
05:01
A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information
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Cited by 1 •
2020
By running the Pathway Association Study Tool (PAST), either through the Shiny application or through the R console, researchers can gain a deeper understanding of the biological meaning of their genome-wide association study (GWAS) results by investigating the metabolic pathways involved.
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
FAQs
Each enzyme catalyzes a particular reaction and influences how quickly its associated step proceeds. Because the product of one step supplies the substrate for the next, changes in enzyme-controlled rates can affect the flow through linked reactions. Regulatory signals further adjust this activity, helping the pathway respond to the cell’s current conditions rather than operating at a fixed rate.
Regulatory signals coordinate pathway activity with cellular needs. They can adjust enzyme-controlled reaction rates so that metabolic activity reflects changing conditions, such as the need to obtain energy or synthesize cellular components. This control helps prevent pathway activity from becoming disconnected from the cell’s requirements and supports the broader biological functions that metabolism must maintain.
Catabolic pathways break down molecules in ways that release energy, with some of that energy captured as ATP or reducing equivalents. Anabolic pathways use energy to construct cellular materials, including proteins, lipids, and nucleic acids. Their opposing purposes connect energy release with biosynthesis, allowing cells to both obtain usable energy and produce the components required for life.
Mapping traces the sequence of linked reactions, the molecules converted at each step, and the enzymes that control those conversions. It can also clarify where regulatory signals influence pathway activity. This organized view helps biologists connect molecular reactions with larger outcomes, such as energy production, construction of cellular components, and maintenance of cellular function.
Researchers use pathway mapping and analysis to examine physiology, disease, drug responses, nutrition, and biotechnology. The approach links individual molecular conversions with broader biological outcomes, making it useful when investigators need to understand how cellular processes change or how a treatment, nutritional condition, or engineered system relates to metabolism.
Metabolic pathways provide a framework for connecting enzyme activity, energy handling, biosynthesis, and cellular regulation. In biology, this framework supports interpretation of how organisms obtain energy, produce proteins, lipids, and nucleic acids, and maintain life. It also allows findings about cellular reactions to inform studies of physiology, disease, nutrition, and biotechnology.