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Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
JoVE Journal
Biology
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JoVE Journal Biology
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

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07:26 min

January 26, 2012

DOI:

07:26 min
January 26, 2012

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Summary

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13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.

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