Mouse Imaging
Mouse Imaging - Related Videos
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
09:52
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
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Cited by 25 •
2013
Recently developed imaging techniques using near-infrared fluorescence (NIRF) may help elucidate the role the lymphatic system plays in cancer metastasis, immune response, wound repair, and other lymphatic-associated diseases.
10:02
High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
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Cited by 6 •
2015
This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.
07:46
In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
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Cited by 39 •
2010
Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.
03:07
Bioluminescence Imaging of Neuroinflammation in a Transgenic Mouse
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2025
Source: Breid, S., et. al. Bioluminescence Imaging of Neuroinflammation in Transgenic Mice After Peripheral Inoculation of Alpha-Synuclein Fibrils. J. Vis. Exp. (2017)This video demonstrates the bioluminescence imaging of neuroinflammation in transgenic mice, showcasing luciferase-luciferin reactions in activated astrocytes. The protocol enables non-invasive quantification of astrocytic activation, aiding studies on CNS inflammation and therapeutic efficacy.
FAQs
These optical approaches make labeled cells or molecular signals visible, allowing researchers to follow where those signals occur and how they change during an experiment. Their value lies in linking a biological process to a detectable visual readout. This is especially useful when the research question focuses on cell behavior, signaling, or disease-related molecular activity rather than anatomy alone.
The biological target guides modality selection. Magnetic resonance imaging can reveal soft tissues, ultrasound can provide information about blood flow, and micro-computed tomography can show skeletal structure. Fluorescence and bioluminescence are more appropriate when labeled cells or molecular signals are the focus. Matching the technique to the target helps produce information relevant to the experimental question.
Following the same animal over time supports longitudinal analysis of development, disease progression, or therapeutic response. Because measurements come from the same mouse, researchers can examine changes within an individual rather than relying only on differences between separate groups. This design can reduce experimental variability and reveal how a biological process evolves across multiple observations.
Researchers should begin with the feature they need to observe, such as labeled cells, molecular signals, soft tissue, blood flow, or skeletal structure. They can then select fluorescence, bioluminescence, magnetic resonance imaging, ultrasound, or micro-computed tomography according to that target. The choice also depends on whether the study examines living animals repeatedly or preserved specimens at a defined stage.
A study typically starts by defining the biological question and identifying the structure, signal, or process requiring visualization. Investigators then select a compatible imaging modality, examine living or preserved mice as appropriate, and compare the resulting observations across conditions or time points. This workflow connects visual measurements with questions about development, disease progression, or treatment response.
Mouse Imaging contributes to research on development, cancer, neuroscience, immunology, and therapeutic responses. Depending on the selected technique, results may show structural changes, skeletal features, blood-flow information, labeled-cell distribution, or molecular signals. Repeated observations can help investigators relate these findings to disease progression or treatment effects while preserving a time-based view of the biological process.