Mouse Liver Model
Mouse Liver Model - Related Videos
06:26
Mouse Model of Metabolic Dysfunction-Associated Steatotic Liver Disease with Fibrosis
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Cited by 1 •
2025
Mouse model of metabolic dysfunction-associated steatotic liver disease (MASLD) with metabolic dysfunction, hepatic gene expression changes, and liver histopathological alterations that resemble human MASLD, including fibrosis that progresses to advanced fibrosis stage 3. This model can be used in studies of MASLD pathophysiology and in pre-clinical studies of new therapies.
03:06
Developing a Humanized Mouse Model with Human Liver Cells and Immune Cells
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2025
This video demonstrates a method for generating a dual humanized mouse model with a human immune system and liver cells. It involves transplanting human liver cells and hematopoietic stem and progenitor cells into a transgenic immunodeficient mouse via intrasplenic injection, leading to engraftment of liver cells and development of human immune cells.
00:10
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
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Cited by 7 •
2019
This protocol provides a reliable method to establish humanized mice with both human immune system and liver cells. Dual reconstituted immunodeficient mice achieved via intrasplenic injection of human hepatocytes and CD34+ hematopoietic stem cells are susceptible to human immunodeficiency virus-1 infection and recapitulate liver damage as observed in HIV-infected patients.
06:35
Generating a Double Humanized BLT (Bone-Marrow, Liver, Thymus) Mouse Model
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2025
This video demonstrates the method for generating a double humanized bone BLT (marrow, liver, and thymus) mouse with a functional human immune system and a stable engrafted human-like gut microbiome.
11:44
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
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Cited by 3 •
2016
Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.
FAQs
Living mice provide whole-organ outcomes, whereas isolated liver tissue and cultured mouse hepatocytes offer more focused experimental systems. This distinction affects the level of biology researchers can examine: whole animals connect hepatic changes to the organism, while tissue or cell preparations help evaluate hepatocyte behavior and molecular responses under defined manipulations. Selecting the format therefore aligns the model with the research question.
Researchers can manipulate genes or administer defined compounds, induce controlled injury, and then measure hepatocyte behavior, metabolism, inflammation, and tissue repair. Comparing these responses links an experimental change to observable hepatic effects. This multilevel design is valuable because it can show how molecular or cellular disturbances develop into broader changes in liver function and recovery.
Each intervention addresses a different experimental question. Genetic manipulation helps test how a selected gene contributes to hepatic processes, whereas controlled injury provides a reproducible challenge for examining inflammation and tissue repair. Defined compounds add a way to study responses to specific exposures. Together, these approaches let investigators attribute measured changes to an intentional experimental condition.
Mouse results can reveal mechanisms and whole-organ responses, but mice and humans differ biologically. Those differences may affect how liver cells behave, process compounds, respond to disease, or repair injury. Consequently, findings should be interpreted as evidence from a controlled biological system and considered carefully before being translated to human biology.
An experiment generally begins by selecting living mice, isolated liver tissue, or cultured mouse hepatocytes according to the research question. Researchers then apply a genetic manipulation, defined compound, or controlled injury and measure changes in hepatocyte behavior, metabolism, inflammation, or tissue repair. Comparing experimental conditions allows the biological effect of the intervention to be assessed.
These systems support studies of drug metabolism and toxicity, viral and metabolic liver diseases, fibrosis, regeneration, and cancer. The same general strategy can examine how the liver processes compounds, responds to disease-related conditions, develops injury, or repairs tissue. Measurements of cellular behavior and metabolism can be interpreted alongside inflammation and tissue-repair responses to characterize disease mechanisms.
Within biology, the model provides a way to connect hepatic structure and function with genetic and cellular events. Genetic analysis can identify relationships between altered genes and liver phenotypes, while measurements of hepatocytes, metabolism, inflammation, and repair show how those relationships appear in tissue. This combination helps explain organ-level processes rather than examining molecular events in isolation.