Msc Marker Expression
Msc Marker Expression - Related Videos
11:13
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
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Cited by 47 •
2017
Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.
11:05
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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Cited by 21 •
2017
The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
04:49
MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model
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2025
This video describes the technique of seeding mesenchymal stem cells (MSCs) onto a biodegradable poly(lactic acid) (PLA) scaffold. The prepared scaffolds can be implanted into the surgical resection cavity of the brain from where they may migrate towards tumor foci. Further, these cells can be engineered to express therapeutic proteins to stimulate apoptosis in cancer cells.
06:56
Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium
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Cited by 4 •
2015
Mitochondrial dysfunction is a hallmark of cellular senescence. This paper uses non-invasive near-infrared (NIr) treatment to improve mitochondrial function in the aging mouse vestibular sensory epithelium.
03:19
Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro
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2023
The following video describes a technique of transduction to label (patient-derived xenografts) PDX tumor cells, which is used for introducing lentivirus expressing green-fluorescent protein and luciferase reporters into the tumor cell in vitro.
FAQs
Interpretation depends on the combined pattern, not a single marker. A profile that includes CD73, CD90, and CD105 together with absence of hematopoietic markers provides phenotypic evidence for classifying an MSC-like population. Using positive and negative features together helps distinguish the target population from other cells without implying that one detected surface protein identifies the cells alone.
Marker profiles are informative for phenotypic classification and assessment of biological state, but they do not by themselves prove stemness or functional potency. This distinction matters because cells may display the expected surface pattern without demonstrating the functional properties required in a differentiation study, regenerative medicine strategy, or cell-based therapy. Functional conclusions therefore require additional evaluation.
To compare tissue sources, researchers can examine whether cell populations show comparable patterns of CD73, CD90, and CD105 and comparable absence of hematopoietic markers. The value of this comparison comes from applying a consistent phenotypic framework across samples. Marker expression supports source-to-source characterization, while the profile should not be treated as a complete measure of biological performance.
Flow cytometry, immunostaining, and related assays provide ways to measure the selected surface-marker profile. The workflow centers on assessing expression of CD73, CD90, and CD105 while checking for absence of hematopoietic markers. The resulting pattern can support phenotypic classification, provided researchers interpret the assay readout as a profile rather than a single-marker result.
Marker-based characterization can support cell isolation by helping researchers identify populations with the desired phenotypic pattern and distinguish them from other cells. Selection criteria can include both the expected MSC-associated markers and the absence of hematopoietic markers. This provides a consistent basis for defining the population selected for subsequent biological studies and characterization.
During culture quality assessment, researchers can use the marker profile to check whether a cell preparation retains the phenotype expected for the study. This information helps document the biological state of cultured cells and compare preparations. It remains a quality indicator, not a standalone demonstration of stemness, potency, or therapeutic performance.
In differentiation studies, regenerative medicine research, and cell-based therapy development, MSC marker expression helps describe and compare the cell populations being evaluated. It can document whether preparations meet a selected phenotypic classification before researchers interpret study outcomes. Because the profile does not establish functional potency alone, conclusions should distinguish surface phenotype from demonstrated biological activity.