Myogenic Progenitor Cells
Myogenic Progenitor Cells - Related Videos
10:03
Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
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Cited by 30 •
2014
Induced pluripotent stem cell (iPSC)-derived myogenic progenitors are promising candidates for cell therapy strategies to treat muscular dystrophies. This protocol describes transplantation and functional measurements required to evaluate the engraftment and differentiation of iPSC-derived mesoangioblasts (a type of muscle progenitors) in mouse models of acute and chronic muscle regeneration.
07:51
Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages
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Cited by 32 •
2014
In vitro culture systems have proven indispensible to our understanding of vertebrate myogenesis. However, much remains to be learned about nonmammalian skeletal muscle development and growth, particularly in basal taxa. An efficient and robust protocol for isolating the adult stem cells of this tissue, the myogenic precursor cells (MPCs), and maintaining their self-renewal, proliferation, and differentiation in a primary culture setting allows for the identification of conserved and divergent...
13:43
Fabrication of Myogenic Engineered Tissue Constructs
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Cited by 11 •
2009
Here, we demonstrate fabrication of collagen-based, tissue constructs containing skeletal myoblasts. These 3-D engineered constructs may be used to replace or repair tissues in vivo. For our purposes, we have designed these as an atrioventricular electrical conduit for the repair of complete heart block[1].
12:55
Purification of Progenitors from Skeletal Muscle
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Cited by 31 •
2011
Method for the enzymatic dissociation, surface labeling and purification by flow cytometry of fibro/adipogenic and myogenic progenitors from murine skeletal muscle.
09:49
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
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Cited by 7 •
2021
This protocol outlines a method to isolate Fibro-adipogenic progenitors (FAPs) and myogenic progenitors (MPs) from rat skeletal muscle. Utilization of the rat in muscle injury models provides increased tissue availability from atrophic muscle for the analysis and a larger repertoire of validated methods to assess muscle strength and gait in free-moving animals.
FAQs
Activation and lineage commitment determine whether a precursor population contributes to muscle formation. Developmental signals or tissue injury can activate these cells, after which they proliferate and commit to the myogenic lineage. This staged progression matters because cell expansion increases the available population, while commitment directs that population toward producing muscle rather than remaining in an earlier precursor state.
Self-renewal allows myogenic progenitor cells to persist as a replenishable source during muscle biology. In the context of satellite-cell activity, this property supports continued study of how muscle maintains or restores its precursor population. Comparing self-renewal with differentiation helps researchers examine whether cells remain available for future muscle formation or enter the pathway toward mature tissue.
Once committed, myogenic progenitor cells can differentiate into myoblasts, which then fuse to form multinucleated myotubes. These structures represent an intermediate stage before mature muscle fibers develop. Following this progression allows researchers to evaluate whether cells have advanced through the myogenic pathway and to connect cellular differentiation with the formation of functional muscle tissue.
During development, developmental signals guide these cells through proliferation, lineage commitment, and muscle formation. After injury, tissue damage activates a related regenerative response involving the same broad progression toward new muscle. Studying both settings helps biology researchers compare how progenitor-cell activity supports normal formation versus repair and regeneration.
Studies of myogenic progenitor cells can support disease modeling by providing a cellular system for examining muscle-related disorders such as muscular dystrophy. Researchers can relate progenitor behavior to stages of muscle formation and regeneration. This makes the cells useful for investigating how disease-associated conditions may affect lineage progression, self-renewal, or the formation of mature muscle.
Their capacity for self-renewal and muscle formation makes myogenic progenitor cells relevant to regenerative medicine and tissue engineering. Researchers can use these properties when considering approaches to replace, repair, or model muscle tissue. These applications build on the cells’ ability to progress from precursor states toward myoblasts, myotubes, and mature muscle fibers.