Nanoliter Dispensing
Nanoliter Dispensing - Related Videos
07:05
Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
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Cited by 6 •
2015
A Cartesian bioprinter was designed and fabricated to allow multi-material deposition in precise, reproducible geometries, while also allowing control of environmental factors. Utilizing the three-dimensional bioprinter, complex and viable constructs may be printed and easily reproduced.
09:32
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
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Cited by 66 •
2013
Ice binding proteins (IBPs), also known as antifreeze proteins, inhibit ice growth and are a promising additive for use in the cryopreservation of tissues. The main tool used to investigate IBPs is the nanoliter osmometer. We developed a home-designed cooling stage mounted on an optical microscope and controlled using a custom-built LabVIEW routine. The nanoliter osmometer described here manipulated the sample temperature in an ultra-sensitive manner.
10:14
Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
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Cited by 9 •
2016
This protocol describes a bioprinting methodology using an automated robotic depositing system that incorporates etched topographical guidance cues with the precision deposition of a cell bearing hydrogel bioink. The printed cells are directly delivered to the etched features and are able to sense and orientate with them.
08:06
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
0 Views •
Cited by 1 •
2016
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
10:36
Prescribed 3-D Direct Writing of Suspended Micron/Sub-micron Scale Fiber Structures via a Robotic Dispensing System
0 Views •
Cited by 6 •
2015
Here, we present a protocol to fabricate freely-suspended, micron/sub-micron scale polymer fibers and “web-like” structures generated via automated direct writing procedure by means of a 3-axis dispensing system.
FAQs
Positive-displacement, piezoelectric, and acoustic systems provide different ways to actuate liquid transfer, but the source material does not assign one mechanism to a specific biological application. Their shared functional role is to meter each aliquot while controlling droplet formation, volume, and placement. This flexibility supports automated, miniaturized workflows that require precise liquid handling.
Reliable nanoliter dispensing depends on controlling three linked outcomes: whether a droplet forms correctly, how much liquid it contains, and where it lands. Small errors in any one can affect a miniaturized assay or normalization step. Monitoring these outcomes is therefore central to accurate liquid handling, particularly when samples are scarce or concentrated.
Eliminating conventional pipette tips changes how liquid handling is organized because the dispenser can meter and place aliquots without relying on those tips. This design supports lower sample and reagent consumption and fits automated workflows. The benefit is especially relevant when experiments require many small transfers or when available biological material is limited.
Reagent normalization uses controlled aliquots to make liquid inputs more consistent across biological experiments. Nanoliter dispensing supports this goal by combining accurate volume handling with automated transfer while reducing reagent use. More consistent inputs can support reproducibility in miniaturized assays and higher-throughput workflows without requiring unnecessary quantities of valuable material.
At the operational level, the workflow centers on transferring a biological sample or reagent, metering the intended aliquot, forming the droplet, and placing it at the required location. A specialized dispenser performs these functions through positive displacement, piezoelectric actuation, or acoustic energy. Automation then supports repeated handling in miniaturized or high-throughput experiments.
Nanoliter dispensing is useful when biology workflows demand small volumes, precision, or repeated transfers. Supported applications include miniaturized assays, high-throughput screening, DNA analysis, protein analysis, cell-based experiments, and reagent normalization. These use cases span molecular and cellular work, showing that the approach can support diverse experimental formats rather than a single assay category.
The main practical outcomes are reduced sample and reagent consumption, lower costs, increased experimental throughput, and improved reproducibility. These gains come from accurate, automated handling of very small aliquots rather than from changing the biological assay itself. Consequently, the method is valuable when laboratories need to conserve material while processing many measurements or experimental conditions.