Neuron Membrane
Neuron Membrane - Related Videos
09:27
Cell Subtype-specific Analysis of Neuronal Membrane Proteasome in Somatosensory Neurons
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2025
The neuronal membrane proteasome (NMP) was recently identified in a subset of somatosensory neurons as a key regulator of touch, pain, and itch perception. This article presents a robust workflow for analyzing cell-type-specific NMP expression and function using cell sorting, transcriptome profiling, and culture-based immunofluorescent analyses.
03:44
Co-Culture of Schwann Cells with DRG Neurons on a Pre-Stretched Membrane
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2025
The video demonstrates the co-culturing of dorsal root ganglion (DRG) neurons with Schwann cells. DRG neurons are first grown on a pre-stretched membrane for aligned axon growth. Schwann cells are then introduced, which attach to the axons, insulating them and promoting neural cell survival and function.
10:18
Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
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Cited by 3 •
2017
Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.
02:19
Immunolabeling of Neuronal Membrane Proteins in a Freeze-fractured Specimen of Mouse Brain Tissue
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2025
Source: Schönherr, S., et. al. Combined Optogenetic and Freeze-fracture Replica Immunolabeling to Examine Input-specific Arrangement of Glutamate Receptors in the Mouse Amygdala. J. Vis. Exp. (2016)This video demonstrates the immunolabelling of freeze-fractured replicas of mouse brain tissue for electron microscopy. The fractured and coated replicas are digested to expose receptors. Primary and gold-tagged secondary antibodies are added to label specific neuronal membrane receptors. The replica...
12:58
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
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Cited by 8 •
2012
By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.
FAQs
The electrochemical gradient establishes a difference in charged particles across the membrane, especially sodium and potassium. Membrane proteins regulate these particles and help maintain that difference. When stimulation reaches the appropriate threshold, the resulting change in membrane potential supports the rapid electrical signaling required for communication along the axon.
A threshold stimulus initiates coordinated ion flow through membrane proteins, producing a rapid change in membrane potential. This response differs from weaker stimulation because it reaches the level required to generate an action potential. Once generated, the electrical signal travels along the axon, allowing the neuron to transmit information.
Ion channels, pumps, and receptors contribute to membrane signaling in different ways. Channels and pumps regulate the movement of charged particles, including sodium and potassium, while receptors participate in detecting or responding to stimulation. Together, these proteins influence the electrochemical gradient and determine how membrane activity affects neuronal communication.
Changing the activity of ion channels, pumps, or receptors can modify the movement of charged particles across the neuron membrane. That change may affect the electrochemical gradient, membrane potential, or ability to reach threshold. Because these processes control action potential generation, altered protein activity can change how neurons communicate with their environment.
A focused study can examine the movement of sodium and potassium, the electrochemical gradient, changes in membrane potential, and the generation or travel of action potentials. Researchers can also consider how membrane proteins respond to stimulation. Together, these observations connect molecular membrane activity with electrical communication along the neuron.
The membrane provides a way to connect environmental stimulation with neuronal signaling. Researchers can examine how receptors and ion-regulating proteins respond, whether membrane potential reaches threshold, and how an action potential travels along the axon. These relationships help explain how neurons participate in sensory processing and broader nervous system function.
Neurological disorders and drug effects can be studied by examining processes that regulate membrane signaling. In particular, researchers can assess whether altered ion channel or receptor activity changes charged-particle movement, membrane potential, or action potential generation. This approach links molecular effects at the neuron membrane with changes in neuronal communication and nervous system activity.