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Developmental Biology

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Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Article DOI: 10.3791/55297 10:18 min June 26th, 2017
June 26th, 2017



Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.



Biosensing Motor Neuron Membrane Potential Live Zebrafish Embryos FRET-based GEVI Excitable Cells Noninvasive Physiological Conditions Neurological Disorders Genetically-encoded Voltage Indicators Spinal Neuron Excitability In-vitro Systems In-vivo Systems Imaging Studies PCR Mixture Mermaid Biosensor Coding Sequence Gel Purification Kit DNA Fragment PCMV-SC Blunt Vector SmaI Restriction Enzyme
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