Oncogenic Pathways
Oncogenic Pathways - Related Videos
03:07
Immortalizing Endothelial Cells via Retroviral Transduction of Viral Oncogenes
0 Views •
2026
Source: McAllister, S. C., Hanson, R. S., et al. An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus. J. Vis. Exp.(2017)This video demonstrates retroviral transduction of primary endothelial cells with HPV16 E6/E7 oncogenes. The integrated oncogenes inactivate p53 and Rb tumor suppressors, extending cell lifespan. G418 selection enriches for transduced cells, yielding immortalized endothelial cultures suitable for transformation...
01:33
Cancer-Critical Genes I: Proto-oncogenes
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2023
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype. When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors. Such genes that act...
01:33
Cancer-Critical Genes I: Proto-oncogenes
0 Views •
2021
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype. When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors. Such genes that act...
08:18
Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene
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Cited by 23 •
2014
The Focus Formation Assay provides a straightforward method to assess the transforming potential of a candidate oncogene.
01:27
C4 Pathway and CAM
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2019
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy. C4 Pathway The C4 pathway is used by plants such as...
FAQs
These changes can activate signals that promote growth, disable apoptosis, weaken cell-cycle checkpoints, or reprogram metabolism. Each effect removes a control that normally limits abnormal-cell expansion or persistence. Considering these changes together helps explain why a tumor can both proliferate excessively and survive conditions that would eliminate properly regulated cells.
Examining these pathways together places tumor behavior in the context of interconnected signaling rather than a single molecular route. Their analysis can help researchers relate altered growth and survival signals to tumor characteristics, identify potential vulnerabilities, and interpret why blocking one signaling component may not fully account for how a tumor grows or persists.
Interactions among signaling networks can give tumor cells multiple ways to maintain growth or survival when treatment disrupts one route. This systems-level view helps explain therapeutic resistance, because a tumor may not depend on an isolated pathway in practice. Studying those interactions supports combination strategies designed to address more than one vulnerability at the same time.
Pathway mapping organizes information about altered growth, survival, division, and transformation signals so researchers can connect molecular changes with tumor behavior. In cancer research, examining pathways such as RAS-MAPK and PI3K-AKT can reveal candidate biomarkers and vulnerabilities. The resulting map supports more informed investigation of diagnosis and treatment rather than focusing on a single alteration in isolation.
Altered pathway activity can provide molecular features that distinguish tumor behavior or indicate a relevant vulnerability. Researchers use pathway knowledge to interpret such features in relation to growth, survival, metabolism, or cell-cycle control. This makes oncogenic pathways useful for identifying biomarkers that may support more precise cancer diagnosis or help guide therapeutic investigation.
Combination strategies are important when tumor behavior reflects several interacting pathway changes rather than one isolated defect. By considering growth-promoting signals alongside survival mechanisms, checkpoint loss, or metabolic reprogramming, researchers can pursue interventions that address complementary vulnerabilities. This approach is especially relevant to therapeutic resistance and to developing more precise treatment strategies.