Organ Culture Filter
Organ Culture Filter - Related Videos
08:45
Whole Organ Tissue Culture
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2023
Whole organs can be cultured ex vivo using specialized bioreactors with the goal of repairing or replacing entire organs. This method uses a donor organ that is stripped of all cells, leaving behind the three-dimensional structure, which is then repopulated with new cells. This video demonstrates the whole organ culture of lungs and shows how a dynamic culture that mimics the mechanical stimulation in the body is needed to induce native tissue properties.
07:50
Preparation of 2-dGuo-Treated Thymus Organ Cultures
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Cited by 9 •
2008
This video demonstrates the dissection and removal of the fetal thymus as well the preparation of ex vivo cultures of 2-dGuo-treated thymus.
09:23
A Rapid Filter Insert-based 3D Culture System for Primary Prostate Cell Differentiation
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Cited by 7 •
2017
Here, we present a method for the establishment of a rapid in vitro system that supports the three dimensional culturing and subsequent luminal differentiation of primary prostate epithelial cells.
01:27
Passive Filters
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2024
Passive filters are utilized to shape the frequency spectrum of signals across a diverse array of applications. These filters, using only passive elements like resistors (R), inductors (L), and capacitors (C), are capable of selectively allowing or blocking certain frequency ranges without the need for external power sources. Low-Pass Filters Low-pass filters are designed to transmit signals with frequencies lower than the cutoff frequency, ωc, and attenuate those above it. The cutoff frequency...
12:29
Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
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Cited by 52 •
2010
This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.
FAQs
The interface keeps the tissue exposed to the culture environment without requiring complete immersion. Medium and dissolved gases can reach the specimen through the porous support, while the tissue remains arranged in three dimensions. This balance helps preserve structural organization during culture and supports subsequent microscopy or functional measurements.
The pores provide routes for culture medium, nutrients, and dissolved gases to move toward the tissue while also supporting waste removal. This transport reduces dependence on direct immersion and helps maintain a controlled local environment. Because the support limits the tissue's contact with bulk liquid, it can retain architecture needed for structure-sensitive observations.
An isolated-cell system separates cells from much of their native tissue arrangement, whereas a filter-supported organ culture retains excised tissue in a three-dimensional organization. That difference allows researchers to examine development, physiology, injury, or treatment responses in a context that more closely reflects tissue structure than many cell-based models.
Preserving three-dimensional organization lets investigators relate visible tissue structure to functional behavior rather than examining isolated cellular responses alone. In an Organ Culture Filter setup, microscopy can assess architecture, while functional assays can evaluate how the cultured tissue behaves under selected conditions. This supports development of models for biology and biomedical research.
Researchers begin with an excised organ or tissue section, position it on the porous support, and expose the setup to culture medium under controlled laboratory conditions. The filter maintains the specimen at the air-liquid interface while nutrients, dissolved gases, and waste exchange occur through the pores. Investigators can then perform microscopy or functional assays on the maintained tissue.
At minimum, the system requires an excised organ or tissue section, a porous membrane or support, and culture medium. The culture arrangement must provide a controlled air-liquid interface and permit movement of nutrients, dissolved gases, and wastes. Depending on the study, microscopy and functional-assay tools are added to measure structural or physiological responses.
Researchers may select this approach when a study needs to examine tissue development, physiology, injury, or responses to drugs and other treatments without discarding three-dimensional organization. It is also useful for model development, because the cultured specimen can support both imaging and functional assays. The method therefore links tissue-level structure with experimentally measured outcomes.