Lung Isolation
Lung Isolation - Related Videos
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
09:47
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
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Cited by 3 •
2016
This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.
06:31
Primary Tumor and MEF Cell Isolation to Study Lung Metastasis
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Cited by 13 •
2015
The goal of this protocol is to study breast tumorigenesis. With this technique, mouse mammary tumors are removed and primary cells are prepared from tumors. A lung extraction protocol is included for studying lung metastasis. Furthermore, another protocol for analyzing mouse embryonic fibroblasts from the mouse embryo is included.
08:59
Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program
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Cited by 27 •
2015
Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.
10:02
Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
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Cited by 12 •
2011
The following protocol outlines the process of isolating, ventilating and instrumenting mouse lungs to measure steady or pulsatile pulmonary vascular pressure-flow relationships in order to quantify the effects of blood flow, airflow, airway changes and vascular changes on right ventricular afterload.
FAQs
Its central mechanism is independent airway control: one lung can receive air while the other is kept outside the ventilation pathway. This separation lets clinicians manage each side according to the needs of a thoracic procedure. In research, the same arrangement allows investigators to examine regional differences in lung function, ventilation, and gas exchange rather than treating both lungs as one unit.
A double-lumen endotracheal tube and a bronchial blocker are alternative devices for establishing separate access to the lungs. Either can support selective ventilation and isolation of one side. Because correct positioning determines whether the intended lung is isolated, an anesthesiologist confirms placement with bronchoscopy before relying on the arrangement for surgery or respiratory investigation.
Separating the lungs helps prevent material in one lung from entering the other. This is particularly relevant when blood, pus, or lavage fluid is present, because isolation limits its spread across the airways. During thoracic procedures, stopping ventilation to the operative side also creates a still surgical field, which supports the procedural objective without requiring both lungs to remain active.
The process begins with an anesthesiologist placing either a double-lumen endotracheal tube or a bronchial blocker. Bronchoscopy then confirms that the device is positioned to separate the intended lung. Once placement is verified, ventilation can be controlled independently, allowing one lung to receive air while the other remains isolated for the planned procedure or study.
Clinical use centers on thoracic procedures that benefit from separate control of the lungs and a stable operative field. It is also valuable when material such as blood, pus, or lavage fluid could move from one lung to the other. By limiting that spread and permitting one-lung ventilation, the technique supports procedures where regional separation is important.
In biology and respiratory research, separate control of the lungs makes it possible to study regional lung function, ventilation, and gas exchange. Investigators can also examine how a localized injury affects one lung or how a localized treatment changes respiratory behavior. This regional design provides information that may be obscured when both lungs are evaluated together.