Pancreatic Xenografts
Pancreatic Xenografts - Related Videos
11:44
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
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Cited by 40 •
2010
Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.
04:11
DCE-MRI of Orthotopic Pancreatic Tumor Xenografts: A Method to Assess Microvasculature in a Target Tumor Tissue
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2023
This video describes the technique of DCE-MRI for orthotropic pancreatic tumor xenografts in mice. DCE-MRI is a non-invasive method to analyze microvasculature in a target tissue, and useful to assess vascular response in a tumor following a novel therapy. This method will assist investigators to apply DCE-MRI for orthotopic gastrointestinal cancer mouse models.
03:21
Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model
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2023
This video describes the technique to create a zebrafish patient derived xenograft model. In the sample protocol, we assess the efficiency of the drugs Gemcitabine and Navitoclax on the pancreatic tumor xenograft model.
09:56
Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment
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Cited by 19 •
2019
This protocol describes optimization procedures in a virus-based dual fluorescence-labeled tumor xenograft model using larval zebrafish as hosts. This heterogeneous xenograft model mimics the tissue composition of pancreatic cancer microenvironment in vivo and serves as a more precise tool for assessing drug responses in personalized zPDX (zebrafish patient-derived xenograft) models.
05:49
Generation of an Orthotopic Xenograft of Pancreatic Cancer Cells by Ultrasound-Guided Injection
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Cited by 3 •
2021
We present a protocol to generate a minimally invasive orthotopic pancreatic cancer model by ultrasound-guided injection of human pancreatic cancer cells and the subsequent monitoring of tumor growth in vivo by ultrasound imaging.
FAQs
Immune suppression reduces the host’s ability to reject implanted pancreatic material, giving the graft an opportunity to establish and grow. This condition is central to interpreting the model because tumor behavior reflects interactions within a living animal while avoiding the full immune response that could prevent engraftment. It therefore supports observation of growth, invasion, metastasis, and treatment response.
The implant site changes the biological context surrounding the tumor. Subcutaneous placement provides one setting for observing tumor growth, whereas orthotopic placement positions the graft in the pancreas and can alter interactions with nearby tissues. Because location influences tumor behavior and surrounding tissue relationships, site selection should match whether the study emphasizes growth alone or tissue-specific biology.
Vascularization is important because the implanted tumor must establish within a living system rather than remain an isolated cell preparation. In pancreatic xenografts, vascularization accompanies graft establishment and growth, helping the model retain a tissue-level setting for examining tumor progression and responses to anticancer treatments over the course of an in vivo study.
Pancreatic xenografts place tumor cells or tissue in a living animal, allowing researchers to examine growth, invasion, metastasis, and treatment responses in an in vivo setting. Simplified cell assays do not provide the same surrounding tissue context. This distinction makes xenografts useful when researchers need biological behavior that more closely reflects human pancreatic cancer than an isolated assay can provide.
A study begins by selecting pancreatic tumor cells or tissue and introducing that material into an immunocompromised animal. Researchers then choose a subcutaneous or orthotopic placement, depending on the biological context required. The resulting model can be used to examine tumor growth, invasion, metastasis, and responses to anticancer treatments within a living system.
Researchers choose these models when they need to assess anticancer treatment responses in a living tumor environment. The graft permits observation of how pancreatic tumors grow and behave while treatment is evaluated in vivo. This makes pancreatic xenografts valuable for preclinical drug evaluation, especially when simplified cell-based experiments cannot represent the broader tumor setting.
The models support investigation of several linked features of pancreatic cancer biology, including local growth, invasion into surrounding tissue, metastasis, and responses to anticancer treatments. Their value extends beyond measuring whether a graft enlarges. By preserving a living-system context, they help connect tumor progression with interactions between the graft and its surrounding tissues.