Pds 1000 System
Pds 1000 System - Related Videos
04:01
The Modified Single-working Portal Technique Using Lasso-loop Stitch with Needle for Arthroscopic Subscapularis Repair
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2025
We propose a modified arthroscopic technique of suturing the subscapular using a needle-through-line Lasso-loop through a unilateral anterior portal.
06:34
Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato
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Cited by 159 •
2009
Description of a virus-induced gene silencing (VIGS) method for knock-down of gene expression in Nicotiana benthamiana and tomato.
08:32
Development of Whispering Gallery Mode Polymeric Micro-optical Electric Field Sensors
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Cited by 7 •
2013
A high-sensitivity photonic micro sensor was developed for electric field detection. The sensor exploits the optical modes of a dielectric sphere. Changes in the external electric field perturb the sphere morphology leading to shifts in its optical modes. The electric field strength is measured by monitoring these optical shifts.
10:15
Generation of Transgenic C. elegans by Biolistic Transformation
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Cited by 27 •
2010
Transgenic worms are commonly used in C. elegans research. Described is a simple, yet effective, protocol to introduce transgenes into worms using biolistic bombardment with DNA-coated gold particles. The effort involved and results of bombardment compare favorably with microinjection for the generation of transgenic animals.
11:25
Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
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Cited by 21 •
2012
A method for producing Arabidopsis leaf protoplasts that are compatible with fluorescence activated cell sorting (FACS), allowing for studies of specific cell populations. This method is compatible with any Arabidopsis line that expresses GFP in a subset of cells.
FAQs
High-pressure helium provides the force needed to accelerate DNA-coated microparticles toward a biological target. Gold or tungsten particles carry the genetic material, while their momentum allows them to penetrate cell walls or membranes. This physical route helps release DNA inside cells, supporting transformation when biological barriers make other delivery approaches less effective.
The microparticles serve as the moving carrier for the genetic material, and the DNA coating places the intended cargo on their surfaces before acceleration. Gold and tungsten are both used as particle materials in the system. Together, the coating and particle provide a means to move DNA through cellular barriers and into target cells or tissues.
The system can deliver genetic material into cells or tissues for different experimental outcomes. Transient transformation supports analyses in which researchers examine gene expression after delivery, whereas stable transformation supports the development of engineered cells or tissues. This range allows investigators to match the delivery approach to questions about gene function or longer-term genetic engineering.
A basic experiment involves preparing DNA-coated gold or tungsten microparticles, directing them toward the selected cells or tissue, and using high-pressure helium to accelerate them. After impact, the particles penetrate cellular barriers and release DNA inside the target. Researchers then examine the resulting transformation, gene expression, or engineered biological material.
Researchers turn to the PDS 1000 System when cells or tissues are difficult to transform by other methods. Its ability to propel genetic material through cell walls or membranes makes it useful for experimental gene transformation in plant biology and biotechnology. The approach can therefore extend genetic studies to biological materials that present substantial delivery barriers.
Applications include analyzing gene function, studying gene expression, and producing engineered cells or tissues. In plant biology and related biotechnology, the resulting transformations can support functional genomics and genetic engineering studies. The key outcome is access to genetic manipulation in targets that may not respond readily to other transformation methods, broadening experimental options.