Perfusion Cannulation
Perfusion Cannulation - Related Videos
03:44
Langendorff Retrograde Heart Perfusion: An Ex Vivo Technique to Perfuse Isolated Mouse Heart
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2025
In this video, we perform Langendorff retrograde perfusion of an isolated mouse heart. During the procedure, the cardiac activity of the heart is maintained by perfusing it retrogradely with a perfusion solution via the coronary arteries using a cannula inserted into the ascending aorta. The model is used to study the cardiac pathophysiology of the heart.
09:49
Isolation and Cannulation of Cerebral Parenchymal Arterioles
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Cited by 28 •
2016
This manuscript describes a simple and reproducible protocol for isolation of intracerebral arterioles (a group of blood vessels encompassing parenchymal arterioles, penetrating arterioles and pre-capillary arterioles) from mice, to be used in pressure myography, immunofluorescence, biochemistry, and molecular studies.
07:32
Porcine Normothermic Isolated Liver Perfusion
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Cited by 3 •
2023
The porcine model of liver normothermic machine perfusion (NMP), described here, can be successfully used to study NMP as a preservation strategy, a tool for viability assessment, and a platform for organ repair. It holds a high translational value, however it is technically challenging and labor-intensive.
02:05
Intraduodenal Cannulation in a Rat Model for Immunopathology Studies
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2025
This video demonstrates intraduodenal cannulation in a rat model. After anesthetizing the rat, the duodenum is located and a small puncture is created. A J-shaped cannula with a beveled tip is carefully inserted and secured, and the incision is closed. The cannulated rat model enables intra-intestinal infusion for immunopathology studies.
05:18
Cannulation of the Mouse Submandibular Salivary Gland via the Wharton's Duct
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Cited by 17 •
2011
A protocol for the cannulation of the mouse submandibular salivary gland via the Wharton's duct is described. For this experiment, the trypan blue solution is used as a dyer to demonstrate how this technique effectively delivers infusions into the targeted gland, and to suggest the reliability of this new approach as a potential clinical drug/cell therapy for the regeneration of salivary glands.
FAQs
Outcome depends strongly on three linked choices: the cannulation site, the security of the cannula, and the regulation of flow. The site determines where the perfusate enters the preparation, while secure positioning helps maintain access during the experiment. Controlled flow then supports repeatable delivery and waste removal, improving the consistency of biological measurements.
Regulated flow matters because the preparation must receive a sustained, controllable supply rather than an uncontrolled fluid input. Flow conditions help coordinate delivery of nutrients, oxygen, drugs, or experimental solutions with removal of waste products. This control is especially relevant when the goal is to preserve tissue function while comparing physiological responses across experiments.
Perfusate composition determines what the preparation is exposed to during the experiment. Depending on the study, the circulating solution may provide nutrients or oxygen, carry a drug, or serve as another experimental treatment. Because the same access route can also support waste removal, investigators can examine responses under defined conditions while maintaining the biological system outside the body.
A practical workflow begins by selecting an appropriate vessel or tissue compartment, placing the cannula, and securing it so the access point remains stable. Researchers then establish regulated circulation of the chosen perfusate and use the setup for the intended measurements. These steps connect mechanical stability with reliable fluid exchange and help reduce variation between preparations.
Researchers apply perfusion cannulation to organ perfusion, tissue experiments, physiological measurements, and ex vivo studies. These applications differ in the biological preparation and the question being asked, but they share a need for controlled access to the system. The approach is therefore useful when investigators need to maintain or study biological function outside the body.
In ex vivo biology, the method can connect experimental control with physiological observation. A defined perfusate can introduce a nutrient, oxygen source, drug, or other solution, while the setup supports waste removal and measurements from the preparation. This makes the technique relevant for testing how tissues or organs respond under managed conditions rather than relying only on observations inside the body.