Low Co2 Screening
Low Co2 Screening - Related Videos
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
15:29
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
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Cited by 6 •
2016
Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
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Cited by 7 •
2016
This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).
06:40
A Robust Method for the Large-Scale Production of Spheroids for High-Content Screening and Analysis Applications
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Cited by 4 •
2021
This protocol details a method for the production of three different types of spheroids in a manner that makes them suitable for large-scale high-content screening and analysis. In addition, examples are presented showing how they can be analyzed at spheroid and individual cell levels.
FAQs
Reduced CO2 can affect more than carbon availability alone. It may change dissolved inorganic carbon, alter the carbon supplied inside cells, and shift environmental pH. These linked changes can influence growth, metabolism, viability, photosynthetic performance, and gene expression. Screening therefore helps distinguish organisms that remain functional under carbon limitation from those whose activity declines.
pH is an important interpretive variable because reduced carbon dioxide can alter environmental pH along with carbon supply. A change in growth or metabolism may therefore reflect combined carbon-limitation and pH effects rather than a single mechanism. Keeping exposure conditions controlled and comparing them with standard conditions makes these responses easier to evaluate.
The most informative endpoint depends on the biological question. Growth rate indicates whether overall proliferation is maintained, while viability shows whether cells remain alive during exposure. Metabolic measurements address functional activity, photosynthetic performance is relevant when photosynthetic function matters, and gene expression can reveal regulatory responses before major growth changes appear. Several endpoints provide a broader physiological profile.
A standard-condition comparison provides the reference needed to judge whether low-CO2-exposed samples retain, lose, or change function. Researchers can compare growth rate, viability, metabolism, photosynthetic performance, or gene expression between the two conditions. This relative response is more informative than observing a low-CO2 measurement without knowing how the sample behaves under standard conditions.
A basic workflow begins by selecting cells, organisms, or strains and exposing them to controlled low-CO2 conditions. Researchers then measure one or more responses, such as growth, viability, metabolism, photosynthetic performance, or gene expression. They compare these results with measurements from standard conditions and identify samples that preserve the desired biological function under reduced carbon availability.
Researchers can apply this screening when they need to characterize stress tolerance or select robust organisms for research or production. Samples that maintain relevant activity under reduced CO2 may support studies of physiological adaptation or biotechnology settings where carbon availability is not ideal. The approach supports selection based on measured performance rather than assumption.
In biology, low-CO2 screening connects cellular responses with broader questions about carbon availability and ecosystem performance. At the sample level, it can show how carbon limitation affects physiology, photosynthesis, metabolism, or regulation. Across organisms or strains, differences in retained function can help characterize adaptation and indicate which biological systems are more resilient when carbon supply is reduced.